Horigome Satoru, Yoshida Izumi, Tsuda Aiko, Harada Teppei, Yamaguchi Akihiro, Yamazaki Kumiko, Inohana Shuichi, Isagawa Satoshi, Kibune Nobuyuki, Satoyama Toshiya, Katsuda Shin-ichi, Suzuki Shinobu, Watai Masatoshi, Hirose Naoto, Mitsue Takahiro, Shirakawa Hitoshi, Komai Michio
a Laboratory of Nutrition , Graduate School of Agricultural Science, Tohoku University , Sendai , Japan.
Biosci Biotechnol Biochem. 2014;78(5):851-60. doi: 10.1080/09168451.2014.905177. Epub 2014 May 15.
The rhizome of Kaempferia parviflora has been used in traditional Thai medicine. In this study, we identified and compared specific compounds from the hexane extract of K. parviflora with those from other Zingiberaceous plants by using gas chromatography-mass spectrometry. We identified 5,7-dimethoxyflavone (DMF), 5-hydroxy-3,7,3',4'-tetramethoxyflavone (TMF), estimated 3,5,7-trimethoxyflavone, 5-hydroxy-7,4'-dimethoxyflavone, 3,5,7,4'-tetramethoxyflavone, and investigated their anti-inflammatory effects in rat basophilic leukemia (RBL-2H3) cells stimulated with an IgE antigen or a calcium ionophore. We found that DMF and TMF more potently inhibited antigen-induced degranulation than did nobiletin, a well-known anti-inflammatory agent. In addition, compared to RBL-2H3 cells stimulated with a calcium ionophore, those treated with DMF and TMF showed more marked inhibition of the degranulation and the production and mRNA expression of inflammatory mediators. These results suggest that DMF and TMF inhibit an early step in the high-affinity IgE receptor signaling cascade rather than intracellular calcium release and protein kinase C activation.
小花山柰的根茎已被用于传统泰国医学。在本研究中，我们通过气相色谱 - 质谱联用技术，鉴定并比较了小花山柰己烷提取物中的特定化合物与其他姜科植物中的化合物。我们鉴定出了5,7 - 二甲氧基黄酮（DMF）、5 - 羟基 - 3,7,3',4' - 四甲氧基黄酮（TMF）、估算的3,5,7 - 三甲氧基黄酮、5 - 羟基 - 7,4' - 二甲氧基黄酮、3,5,7,4' - 四甲氧基黄酮，并研究了它们在经IgE抗原或钙离子载体刺激的大鼠嗜碱性白血病（RBL - 2H3）细胞中的抗炎作用。我们发现，与著名的抗炎剂川陈皮素相比，DMF和TMF能更有效地抑制抗原诱导的脱颗粒。此外，与经钙离子载体刺激的RBL - 2H3细胞相比，用DMF和TMF处理的细胞对脱颗粒以及炎症介质的产生和mRNA表达表现出更显著的抑制作用。这些结果表明，DMF和TMF抑制了高亲和力IgE受体信号级联反应的早期步骤，而非细胞内钙释放和蛋白激酶C激活。
