School of Cosmetic Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.
Phytocosmetics and Cosmeceuticals Research Group, Mae Fah Luang University, Chiang Rai 57100, Thailand.
Molecules. 2022 Jul 8;27(14):4401. doi: 10.3390/molecules27144401.
(Black ginger) is used widely in medical fields as an anti-microorganism and anti-inflammation. In this study, the aim was to evaluate the in vitro and in vivo anti-acne efficacy of black ginger extract. The results indicate that the methanol and ethanol extracts showed the highest total phenolic contents, without a significant difference, whereas the -hexane extract showed the highest total flavonoid content. Nine flavones were detected using UPLC-QTOF-MS, and the ethyl acetate extract showed the highest amount of 5,7-dimethoxyflavone (DMF) according to HPLC. Antibacterial activity against , , and was observed. All the extracts showed antimicrobial activity against C. acnes, revealing MICs in the range of 0.015 to 0.030 mg/mL, whereas the ethyl acetate extract inhibited the growth of with a MIC of 3.84 mg/mL. In addition, the ethyl acetate extract showed the highest activity regarding nitric oxide inhibition (IC50 = 12.59 ± 0.35 µg/mL). The ethyl acetate extract was shown to be safe regarding cell viability at 0.1 mg/mL. The anti-acne efficacy was evaluated on volunteers. The volunteers were treated in two groups: one administered a 0.02% ethyl acetate extract gel-cream (n = 9) and one administered a placebo (n = 9) for 6 weeks. The group treated with the gel-cream containing the extract showed 36.52 and 52.20% decreases in acne severity index (ASI) after 4 and 6 weeks, respectively, and 18.19 and 18.54% decreases in erythema, respectively. The results suggest that could be a potent active ingredient in anti-inflammatory and anti-acne products.
（黑姜）在医学领域被广泛用作抗菌和抗炎剂。本研究旨在评估黑姜提取物的体外和体内抗痤疮功效。结果表明，甲醇和乙醇提取物的总酚含量最高，无显著差异，而正己烷提取物的总黄酮含量最高。采用 UPLC-QTOF-MS 检测到 9 种黄酮类化合物，根据 HPLC 结果，乙酸乙酯提取物中 5,7-二甲氧基黄酮（DMF）含量最高。该提取物对 、 、 和 表现出抗菌活性。所有提取物均对 C. acnes 表现出抗菌活性，其 MIC 范围为 0.015 至 0.030 mg/mL，而乙酸乙酯提取物对 的 MIC 为 3.84 mg/mL。此外，乙酸乙酯提取物在抑制一氧化氮生成方面表现出最高的活性（IC50 = 12.59 ± 0.35 µg/mL）。乙酸乙酯提取物在 0.1 mg/mL 时对细胞活力的安全性最高。在志愿者中评估了抗痤疮功效。志愿者分为两组：一组使用 0.02%乙酸乙酯提取物凝胶霜（n = 9），另一组使用安慰剂（n = 9）治疗 6 周。使用含有提取物的凝胶霜治疗的组在第 4 和第 6 周时痤疮严重程度指数（ASI）分别降低了 36.52%和 52.20%，红斑分别降低了 18.19%和 18.54%。结果表明，黑姜可能是抗炎和抗痤疮产品中的有效成分。
