Food and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom 73170, Thailand.
The Center for Veterinary Diagnosis, Faculty of Veterinary Science, Mahidol University, Salaya, Phutthamonthon, Nakhon Pathom 73170, Thailand.
Nutrients. 2023 Feb 22;15(5):1098. doi: 10.3390/nu15051098.
The existence of neuroinflammation and oxidative stress surrounding amyloid beta (Aβ) plaques, a hallmark of Alzheimer's disease (AD), has been demonstrated and may result in the activation of neuronal death and inhibition of neurogenesis. Therefore, dysregulation of neuroinflammation and oxidative stress is one possible therapeutic target for AD. Wall. ex Baker (KP), a member of the Zingiberaceae family, possesses health-promoting benefits including anti-oxidative stress and anti-inflammation in vitro and in vivo with a high level of safety; however, the role of KP in suppressing Aβ-mediated neuroinflammation and neuronal differentiation has not yet been investigated. The neuroprotective effects of KP extract against Aβ have been examined in both monoculture and co-culture systems of mouse neuroectodermal (NE-4C) stem cells and BV-2 microglia cells. Our results showed that fractions of KP extract containing 5,7-dimethoxyflavone, 5,7,4'-trimethoxyflavone, and 3,5,7,3',4'-pentamethoxyflavone protected neural stem cells (both undifferentiated and differentiated) and microglia activation from Aβ-induced neuroinflammation and oxidative stress in both monoculture and co-culture system of microglia and neuronal stem cells. Interestingly, KP extracts also prevented Aβ-suppressed neurogenesis, possibly due to the contained methoxyflavone derivatives. Our data indicated the promising role of KP in treating AD through the suppression of neuroinflammation and oxidative stress induced by Aβ peptides.
淀粉样蛋白 β (Aβ) 斑块周围存在神经炎症和氧化应激，这是阿尔茨海默病 (AD) 的标志，可能导致神经元死亡的激活和神经发生的抑制。因此，神经炎症和氧化应激的失调是 AD 的一个可能的治疗靶点。
Wall. ex Baker (KP)，姜科的一员，具有促进健康的益处，包括在体外和体内的抗氧化应激和抗炎作用，而且安全性高；然而，KP 在抑制 Aβ 介导的神经炎症和神经元分化中的作用尚未得到研究。KP 提取物对 Aβ 的神经保护作用已在小鼠神经外胚层 (NE-4C) 干细胞和 BV-2 小胶质细胞的单核培养和共培养系统中进行了研究。
我们的结果表明，KP 提取物的含有 5,7-二甲氧基黄酮、5,7,4'-三甲氧基黄酮和 3,5,7,3',4'-五甲氧基黄酮的部分能够保护神经干细胞（未分化和分化）和小胶质细胞激活免受 Aβ 诱导的神经炎症和氧化应激，无论是在单核培养还是在小胶质细胞和神经元干细胞的共培养系统中。有趣的是，KP 提取物还防止了 Aβ 抑制的神经发生，这可能是由于其包含的甲氧基黄酮衍生物。
我们的数据表明，KP 通过抑制 Aβ 肽诱导的神经炎症和氧化应激，在治疗 AD 方面具有广阔的应用前景。
