超高效液相色谱-质谱法测定炮制绞股蓝中四种达玛烷型皂苷
Determination by UPLC-MS of four dammarane-type saponins from heat-processed Gynostemma pentaphyllum.
作者信息
Wu Qian, Jang Moonhee, Piao Xiang-Lan
机构信息
a Institute of Chinese Minority Traditional Medicine, Minzu University of China , Beijing , China.
出版信息
Biosci Biotechnol Biochem. 2014;78(2):311-6. doi: 10.1080/09168451.2014.882751. Epub 2014 Apr 14.
Heat-processed Gynostemma pentaphyllum and its main dammaran-type saponins, gypenoside L, gypenoside LI, damulin B, and damulin A, possess non-small cell lung carcinoma A549 cell inhibitory activity. We established in this study a method by ultra-high performance liquid chromatography with tandem mass spectrometry for determination of the saponins and also investigated their content change in heat-processed G. pentaphyllum. The main saponins increased with increasing heating temperature and time. Further investigation showed that they were produced from gypenoside XLVI and gypenoside LVI by undergoing hydrolysis during the heat treatment.
炮制后的绞股蓝及其主要达玛烷型皂苷，绞股蓝皂苷L、绞股蓝皂苷LI、达玛林B和达玛林A，具有非小细胞肺癌A549细胞抑制活性。我们在本研究中建立了一种超高效液相色谱-串联质谱法测定皂苷，并研究了它们在炮制绞股蓝中的含量变化。主要皂苷随加热温度和时间的增加而增加。进一步研究表明，它们是由绞股蓝皂苷XLVI和绞股蓝皂苷LVI在热处理过程中发生水解产生的。
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