Institute of Biotechnology, Hue University, Hue 49000, Vietnam.
Vo Nguyen Giap Gifted High School, Quang Binh 47000, Vietnam.
Molecules. 2022 May 6;27(9):2972. doi: 10.3390/molecules27092972.
( (Thunb.) Makino) is used in Northeast and Southeast Asia countries for the treatment of various diseases, including hepatitis, diabetes, and cardiovascular disease. saponins (gypenosides) are the major components responsible for the pharmacological activities. In this study, different concentrations of abiotic (25-200 μM methyl jasmonate-MeJA and salicylic acid-SA) or biotic elicitors (1-5 g/L yeast extract-YE and biomass) were used as plant elicitors, in order to investigate their influences on cell growth and gypenosides accumulation in suspension cells. Suspension cells were grown on a MS medium containing 2.0 mg/L KIN and 0.5 mg/L IBA, with initial inoculum sizes of 3 g and shaking speeds of 120 rpm for 18 days. Gypenoside and Rb1 contents were measured by colorimetric and HPLC methods. Among three elicitors, SA was suitable for gypenosides accumulation in individual treatment. The cell biomass had the same values in elicitated and control suspension cells. Gypenosides content in cells treated with 100 μM salicylic acid after 6 days of culture reached a maximum value of 79.721 mg gypenoside/g dry biomass (including 0.093 mg ginsenoside Rb1/mg dry weight), which was 2.18-folds higher than that of the natural product. The elicitation promises an efficiency strategy for the production gypenosides in suspension cells.
绞股蓝（(Thunb.) Makino）在东北亚和东南亚国家被用于治疗各种疾病，包括肝炎、糖尿病和心血管疾病。三萜皂苷（绞股蓝皂苷）是其发挥药理活性的主要成分。本研究采用不同浓度的非生物（25-200 μM 茉莉酸甲酯-MeJA 和水杨酸-SA）或生物（1-5 g/L 酵母提取物-YE 和生物质）作为植物诱导剂，以研究它们对悬浮细胞生长和绞股蓝皂苷积累的影响。悬浮细胞在含有 2.0 mg/L KIN 和 0.5 mg/L IBA 的 MS 培养基中培养，初始接种量为 3 g，摇床转速为 120 rpm，培养 18 天。采用比色法和 HPLC 法测定绞股蓝皂苷和 Rb1 含量。在三种诱导剂中，SA 适合于单独处理时绞股蓝皂苷的积累。诱导和对照悬浮细胞中的细胞生物量具有相同的值。用 100 μM 水杨酸处理 6 天后，细胞中的绞股蓝皂苷含量达到最大值 79.721 mg 绞股蓝皂苷/g 干生物量（包括 0.093 mg 人参皂苷 Rb1/mg 干重），是天然产物的 2.18 倍。该诱导方法有望成为悬浮细胞中绞股蓝皂苷生产的有效策略。
