Center on Translational Neuroscience and School of Pharmacy, Minzu University of China, Beijing, China.
School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, China.
Nat Prod Res. 2021 Nov;35(22):4433-4441. doi: 10.1080/14786419.2020.1723093. Epub 2020 Feb 10.
Heat-processed has shown strong activity against human lung carcinoma A549 cells. In this study, two dammarane-type saponins together with two known compounds were isolated from the ethanol extract of the heat-processed leaves of . They were identified as 2,3,12-trihydroxydammar-20(22),24-diene-3---D-glucopyranoside (, namely damulin E), 2,3,12-trihydroxydammar-20,24-diene-3---D-glucopyranoside (, namely damulin F), damulin A () and damulin B (), respectively, using NMR and mass spectra. Damulin E and damulin F showed moderate activity against A549, H1299, T24, SH-SY5Y and K562 cell lines in vitro using CCK-8 assay.
热处理后的叶子提取物中分离得到两种达玛烷型皂苷和两种已知化合物。它们被鉴定为 2,3,12-三羟基达玛-20(22),24-二烯-3---D-吡喃葡萄糖苷（，即达玛林 E），2,3,12-三羟基达玛-20,24-二烯-3---D-吡喃葡萄糖苷（，即达玛林 F），达玛林 A（）和达玛林 B（），分别使用 NMR 和质谱。CCK-8 法体外实验结果表明，达玛林 E 和达玛林 F 对 A549、H1299、T24、SH-SY5Y 和 K562 细胞系均有中等活性。
