Nakayama Yoshitaka, Hirata Aiko, Iida Hidetoshi
Department of Biology, Tokyo Gakugei University, Koganei-shi, Tokyo, Japan.
FEMS Yeast Res. 2014 Sep;14(6):992-4. doi: 10.1111/1567-1364.12181. Epub 2014 Jul 22.
The mechanosensitive channels, Mys1 and Msy2, in fission yeast are localized in the endoplasmic reticulum membrane and control cytoplasmic Ca(2+) levels in the hypoosmotic response. We here investigated changes in organellar structures with hypoosmotic shock using transmission electron microscopy. While msy1(-) and msy2(-) single mutant cells developed a number of swollen vacuoles following hypoosmotic shock, similar to wild-type cells, msy1(-) msy2(-) double mutant cells only had two abnormally large vacuoles and cracks between the inner and outer nuclear membranes. These results suggest that Msy1 and Msy2 may be involved in maintaining vacuole integrity and protecting the nuclear envelope upon hypoosmotic shock and also that these two channels are functionally complementary.
裂殖酵母中的机械敏感通道Mys1和Msy2定位于内质网膜中，并在低渗反应中控制细胞质Ca(2+)水平。我们在此使用透射电子显微镜研究了低渗休克时细胞器结构的变化。虽然msy1(-)和msy2(-)单突变细胞在低渗休克后出现了许多肿胀的液泡，与野生型细胞相似，但msy1(-) msy2(-)双突变细胞只有两个异常大的液泡以及内外核膜之间的裂缝。这些结果表明，Msy1和Msy2可能参与在低渗休克时维持液泡完整性和保护核膜，并且这两个通道在功能上是互补的。
