Basic Marin, Jovicic Milentijevic Maja, Basic David, Basic Hakija
Faculty of Medicine, University of Nis, Nis, Serbia.
Appl Immunohistochem Mol Morphol. 2014 Nov-Dec;22(10):774-6. doi: 10.1097/PAI.0000000000000009.
This paper is dedicated to the description of superheating as a method for antigen retrieval. In our investigation, this antigen retrieval method was used on thermal plate, heating tissue sections at temperature 120° C for 90 minutes. In the research we conducted the superheating method was applied to formalin-fixed paraffin-embedded tissue sections of breast tumors. The following monoclonal antibodies were used: estrogen receptor, progesterone receptor, epithelial membrane antigen, CD34, and Ki-67. With these tested antibodies we had good staining and no loss of tissue sections during the staining process.
本文致力于描述过热作为一种抗原修复方法。在我们的研究中，这种抗原修复方法是在热板上进行的，将组织切片在120°C的温度下加热90分钟。在我们进行的研究中，过热方法应用于乳腺肿瘤的福尔马林固定石蜡包埋组织切片。使用了以下单克隆抗体：雌激素受体、孕激素受体、上皮膜抗原、CD34和Ki-67。使用这些经过测试的抗体，我们获得了良好的染色效果，并且在染色过程中组织切片没有损失。
