Chung K T, Fulk G E, Egan M
Appl Environ Microbiol. 1978 Mar;35(3):558-62. doi: 10.1128/aem.35.3.558-562.1978.
Reduction of seven azo dyes (amaranth, Ponceau SX, Allura Red, Sunset Yellow, tartrazine, Orange II, and methyl orange) was carried out by cell suspensions of predominant intestinal anaerobes. It was optimal at pH 7.4 in 0.4 M phosphate buffer and inhibited by glucose. Flavin mononucleotide caused a marked enhancement of azo reduction by Bacteroides thetaiotaomicron. Other electron carriers, e.g., methyl viologen, benzyl viologen, phenosafranin, neutral red, crystal violet, flavin adenine dinucleotide, menadione, and Janus Green B can replace flavin mononucleotide. These data suggest that an extracellular shuttle is required for azo reduction.
七种偶氮染料(苋菜红、丽春红SX、诱惑红、日落黄、柠檬黄、橙黄II和甲基橙)的还原反应由主要的肠道厌氧菌细胞悬液进行。在0.4M磷酸盐缓冲液中,pH值为7.4时反应最佳,且受葡萄糖抑制。黄素单核苷酸可显著增强嗜热栖热放线菌对偶氮染料的还原作用。其他电子载体,如甲基紫精、苄基紫精、番红精、中性红、结晶紫、黄素腺嘌呤二核苷酸、甲萘醌和詹纳斯绿B可替代黄素单核苷酸。这些数据表明偶氮染料的还原需要细胞外穿梭机制。