使用重组颗粒抗原GRA1、GRA7和可溶性抗原的间接ELISA法用于鸡弓形虫感染血清学诊断的评估。
Evaluation of an indirect ELISA using recombinant granule antigen GRA1, GRA7 and soluble antigens for serodiagnosis of Toxoplasma gondii infection in chickens.
作者信息
Sun Xuefei, Wang Zedong, Li Jiping, Wei Feng, Liu Quan
机构信息
College of Life Science, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, Jilin Province, China.
Military Veterinary Institute, Academy of Military Medical Sciences, Changchun 130122, Jilin Province, China.
出版信息
Res Vet Sci. 2015 Jun;100:161-4. doi: 10.1016/j.rvsc.2015.04.011. Epub 2015 Apr 28.
In the present study, recombinant granule antigen proteins GRA1, GRA7 and Toxoplasma soluble antigens (TSA) were evaluated as potential diagnostic markers for T. gondii infection in chickens by an indirect enzyme-linked immunosorbent assay (ELISA), showing a seroprevalence of 16.4% by GRA1-ELISA, and 15.5% by both GRA7- and TSA-ELISA in chickens. No significant difference was observed in the inconsistent results (P > 0.05), and a substantial agreement was found in the consistent results of the 3 tests (92.7-97.3%). Compared with the reference test Western blot, GRA7-ELISA showed the highest co-positivity and co-negativity rates. Receiver operating characteristic (ROC) analysis revealed a largest area under curve (AUC) of 0.99 (95% CI, 98-1.0), and a highest relative sensitivity (100%) and specificity (97.9%) for a cut-off value of 0.34 in GRA7-ELISA. These results of the present study showed that GRA7 is a potential diagnostic marker for the detection of T. gondii infection in chickens.
在本研究中，通过间接酶联免疫吸附测定（ELISA）评估了重组颗粒抗原蛋白GRA1、GRA7和弓形虫可溶性抗原（TSA）作为鸡弓形虫感染潜在诊断标志物的可能性，结果显示，GRA1-ELISA检测的鸡血清阳性率为16.4%，GRA7-ELISA和TSA-ELISA检测的鸡血清阳性率均为15.5%。不一致结果之间未观察到显著差异（P>0.05），三项检测的一致结果存在高度一致性（92.7-97.3%）。与参考检测方法蛋白质印迹法相比，GRA7-ELISA显示出最高的共阳性率和共阴性率。受试者工作特征（ROC）分析显示，GRA7-ELISA的曲线下面积（AUC）最大，为0.99（95%CI，98-1.0），当临界值为0.34时，相对灵敏度最高（100%），特异性最高（97.9%）。本研究的这些结果表明，GRA7是检测鸡弓形虫感染的潜在诊断标志物。
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