College of Life Science and Engineering, Foshan University, Foshan, Guangdong Province, China.
Military Veterinary Institute Academy of Military Medical Sciences, Changchun, Jilin Province, China.
BMC Vet Res. 2019 Oct 25;15(1):366. doi: 10.1186/s12917-019-2121-4.
Toxoplasma gondii, an intracellular apicomplexan protozoan parasite, can infect almost all warm-blooded animals. The aim of the present study was to investigate T. gondii oocyst-driven infection in pigs, chickens and humans in Jilin province, northeastern China.
The serum samples of pigs, chickens and humans were sampled and tested by indirect enzyme-linked immunosorbent assays (ELISAs) using dense granule antigen GRA7, oocyst-specific protein OWP8, and sporozoite-specific protein CCp5A, respectively. Results showed a prevalence of 16.7% by GRA7-ELISA, and 12.2% by OWP8- and CCp5A-ELISA in pigs; 10.4% by GRA7-ELISA, 13.5% by OWP8-ELISA, and 9.4% by CCp5A-ELISA in chickens; and 14.2% by GRA7-ELISA, 3.6% by OWP8-ELISA, and 3.0% by CCp5A-ELISA in humans. No significant differences were observed between T. gondii seroprevalence in pigs and chickens among the three antigens-based ELISAs (P > 0.05). However, there were significant differences between T. gondii seroprevalence rates in humans (P < 0.05). These findings demonstrated a low prevalence of T. gondii oocyst-driven infection in humans, a medium prevalence in pigs, and a high prevalence in chickens.
The present study demonstrated that different oocyst-driven infection rates in different animal species, which would help to design effective strategies to prevent T. gondii transmission. To our knowledge, this is the first study to differentiate T. gondii infective forms in pigs, chickens and humans in China.
刚地弓形虫是一种细胞内顶复门原虫寄生虫，几乎可以感染所有温血动物。本研究旨在调查中国东北地区吉林省猪、鸡和人感染刚地弓形虫卵囊的情况。
通过间接酶联免疫吸附试验（ELISA）分别使用致密颗粒抗原 GRA7、卵囊特异性蛋白 OWP8 和裂殖体特异性蛋白 CCp5A 对猪、鸡和人血清样本进行了检测。结果显示，GRA7-ELISA 的阳性率为 16.7%，OWP8-ELISA 和 CCp5A-ELISA 的阳性率分别为 12.2%和 12.2%；GRA7-ELISA 的阳性率为 10.4%，OWP8-ELISA 的阳性率为 13.5%，CCp5A-ELISA 的阳性率为 9.4%；GRA7-ELISA 的阳性率为 14.2%，OWP8-ELISA 的阳性率为 3.6%，CCp5A-ELISA 的阳性率为 3.0%。三种抗原 ELISA 法检测猪和鸡的弓形虫血清阳性率无显著差异（P>0.05）。然而，人类的弓形虫血清阳性率存在显著差异（P<0.05）。这些发现表明，人类感染刚地弓形虫卵囊的患病率较低，猪的患病率中等，鸡的患病率较高。
本研究表明，不同动物物种的卵囊感染率不同，这有助于设计有效的策略来预防刚地弓形虫的传播。据我们所知，这是首次在中国区分猪、鸡和人感染刚地弓形虫的感染形式。
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