用于测定活细胞中Ca2+的可见光激发比率发射探针的设计。

Design of ratiometric emission probe with visible light excitation for determination of Ca2+ in living cells.

作者信息

Liu Qiaoling, Du Huizhi, Ren Xiaoze, Bian Wei, Fan Li, Shuang Shaomin, Dong Chuan, Hu Qin, Choi Martin M F

机构信息

Institute of Environmental Science, and School of Chemistry and Chemical Engineering, Shanxi University , Taiyuan 030006, China.

出版信息

Anal Chem. 2014 Aug 19;86(16):8025-30. doi: 10.1021/ac5022002. Epub 2014 Aug 1.

DOI:10.1021/ac5022002

PMID:25054689

Abstract

An organic salt as a fluorescent probe based on intramolecular charge transfer for Ca(2+) determination is developed. Ca(2+) can be detected by ratiometric emission at 490 and 594 nm with an excitation wavelength of 405 nm. This probe is highly selective for Ca(2+) over other divalent metal cations and displays a large Stokes shift of 189 nm that can avoid interference of the excitation light beam and autofluorescence of biological samples. The dissociation constant for Ca(2+) is 2.25 ± 0.47 μM and pertinent to Ca(2+) detection in cellular resting and dynamic states. The probe demonstrates its application in monitoring Ca(2+) in living cells under confocal microscopic imaging.

摘要

开发了一种基于分子内电荷转移的有机盐荧光探针用于测定Ca(2+)。在激发波长为405nm时，可通过490和594nm处的比率发射检测Ca(2+)。该探针对Ca(2+)的选择性高于其他二价金属阳离子，并且具有189nm的大斯托克斯位移，可避免激发光束的干扰和生物样品的自发荧光。Ca(2+)的解离常数为2.25±0.47μM，与细胞静息和动态状态下的Ca(2+)检测相关。该探针在共聚焦显微镜成像下展示了其在监测活细胞中Ca(2+)的应用。