National Institute of Infectious Diseases.
Jpn J Infect Dis. 2014;67(4):282-7. doi: 10.7883/yoken.67.282.
Recently, new genotypes of human adenoviruses (HAdVs) have been reported and many of them have been found to be recombinant forms of different known types of HAdV species D (HAdV-D). The objective of this study was to document the evolutionary features of a novel isolate (HAdV_Chiba_E086/2012) obtained from the eye swab of a patient with conjunctivitis in Japan. Viral DNA was extracted from the isolate to sequence the whole genome by the Sanger method and aligned with available genome sequences of HAdV-Ds. The phylogenetic trees of the nucleotide sequences of the penton base, hexon, and fiber genes and the E3 region showed that HAdV_Chiba_E086/2012 is closest to HAdV genotype 65 (HAdV-GT65), HAdV-48, HAdV-GT60 and HAdV-22 at 98%, 99%, 95% and 98% identity, respectively, suggesting that this isolate is a novel recombinant form to be designated as P65H48F60. Further phylogenetic and recombination analyses of the genome alignment of the new isolate implied that nested recombination events involving HAdV-GT59, GT65, 48, GT60, 22, and some ancestral lineages or their close relatives have shaped its genome. These results showed that HAdV_Chiba_E086/2012 is the first HAdV-48-related HAdV found in Japan, which has the most complicated evolutionary history among the known HAdVs so far.
最近，新型人类腺病毒（HAdV）的基因型已被报道，其中许多为不同已知类型 D 型 HAdV（HAdV-D）的重组形式。本研究的目的是记录从日本结膜炎患者眼拭子中获得的新型分离株（HAdV_Chiba_E086/2012）的进化特征。从分离株中提取病毒 DNA，采用 Sanger 法对全基因组进行测序，并与 HAdV-D 种的现有基因组序列进行比对。五邻体基底、六邻体和纤维基因以及 E3 区核苷酸序列的系统发育树表明，HAdV_Chiba_E086/2012 与 HAdV 基因型 65（HAdV-GT65）、HAdV-48、HAdV-GT60 和 HAdV-22 的同源性分别为 98%、99%、95%和 98%，提示该分离株是一种新的重组形式，被命名为 P65H48F60。对新分离株基因组比对的系统发育和重组分析表明，嵌套重组事件涉及 HAdV-GT59、GT65、48、GT60、22 以及一些祖先谱系或其近亲，这些事件塑造了其基因组。这些结果表明，HAdV_Chiba_E086/2012 是日本发现的首个与 HAdV-48 相关的 HAdV，是目前已知 HAdV 中进化历史最复杂的病毒。
