Department of Microbiology, Fukushima Medical University School of Medicine, Fukushima, Japan.
Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
J Gen Virol. 2011 Jun;92(Pt 6):1251-1259. doi: 10.1099/vir.0.030361-0. Epub 2011 Mar 14.
Human adenovirus type 53 (HAdV-53) has commonly been detected in samples from epidemic keratoconjunctivitis (EKC) patients in Japan since 1996. HAdV-53 is an intermediate virus, containing hexon-chimeric, penton base and fiber structures similar to HAdV-22 and -37, HAdV-37 and HAdV-8, respectively. HAdV-53-like intermediate strains were first isolated from EKC samples in Japan in the 1980s. Here, the complete genome sequences of three such HAdV-53-like intermediate strains (870006C, 880249C and 890357C) and four HAdV-53 strains were determined, and their relationships were analysed. The seven HAdV strains were classified into three groups, 870006C/880249C, 890357C and the four HAdV-53 strains, on the basis of phylogenetic analyses of the partial and complete genome sequences. HAdV strains within the same group showed the highest nucleotide identities (99.87-100.00 %). Like HAdV-53, the hexon loop 1 and 2 regions of 870006C, 880249C and 890357C showed the highest identity with HAdV-22. However, these strains did not show a hexon-chimeric structure similar to HAdV-22 and -37, or a penton base similar to HAdV-37. The fiber genes of 870006C and 880249C were identical to that of HAdV-37, but not HAdV-8. Thus, the three intermediate HAdVs isolated in the 1980s were similar to each other but not to HAdV-53. The recombination breakpoints were inferred by the Recombination Detection Program (rdp) using whole-genome sequences of these seven HAdV and of 12 HAdV-D strains from GenBank. HAdV-53 may have evolved from intermediate HAdVs circulating in the 1980s, and from HAdV-8, -22 and -37, by recombination of sections cut at the putative breakpoints.
人腺病毒 53 型（HAdV-53）自 1996 年以来，在日本流行的角膜结膜炎（EKC）患者的样本中经常被检测到。HAdV-53 是一种中间型病毒，包含与 HAdV-22 和 -37、HAdV-37 和 HAdV-8 分别相似的六邻体嵌合、五邻体基底和纤维结构。1980 年代，日本首次从 EKC 样本中分离出 HAdV-53 样中间株。在这里，确定了三个这样的 HAdV-53 样中间株（870006C、880249C 和 890357C）和四个 HAdV-53 株的完整基因组序列，并对它们的关系进行了分析。根据部分和完整基因组序列的系统发育分析，这七种 HAdV 株分为三组，870006C/880249C、890357C 和四个 HAdV-53 株。同一组内的 HAdV 株显示出最高的核苷酸同一性（99.87-100.00%）。与 HAdV-53 一样，870006C、880249C 和 890357C 的六邻体环 1 和 2 区域与 HAdV-22 具有最高的同一性。然而，这些菌株没有显示出与 HAdV-22 和 -37 相似的嵌合六邻体结构，也没有与 HAdV-37 相似的五邻体基底。870006C 和 880249C 的纤维基因与 HAdV-37 相同，但与 HAdV-8 不同。因此，20 世纪 80 年代分离的三种中间型 HAdV 彼此相似，但与 HAdV-53 不同。使用来自 GenBank 的这七种 HAdV 和 12 种 HAdV-D 株的全基因组序列，通过重组检测程序（rdp）推断重组断点。HAdV-53 可能是由 20 世纪 80 年代循环的中间型 HAdV 以及 HAdV-8、-22 和 -37 通过在假定的断点切割的部分重组而进化而来的。
