Charfi-Kessis Karama, Mansour Wejdene, Ben Haj Khalifa Anis, Mastouri Maha, Nordmann Patrice, Aouni Mahjoub, Poirel Laurent
INSERM U914, South-Paris Medical School, K.-Bicêtre, France; Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives LR99ES27, Faculté de Pharmacie, Monastir, Tunisia.
Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives LR99ES27, Faculté de Pharmacie, Monastir, Tunisia.
Microb Pathog. 2014 Sep;74:20-4. doi: 10.1016/j.micpath.2014.07.003. Epub 2014 Jul 22.
Multidrug-resistant and difficult-to-treat Acinetobacter baumannii may be responsible for nosocomial infections. The production of carbapenem-hydrolyzing class D β-lactamases (CHDLs) and extended-spectrum β-lactamase (ESBLs) of the GES type possessing a carbapenemase activity has been increasingly reported worldwide in A. baumannii. The aim of this study was to analyze the resistance mechanisms of two carbapenem resistant A. baumannii clinical isolates recovered in a neonatology center in the center-east of Tunisia. Two carbapenem resistant A. baumannii isolates were recovered. The first isolate co-harbored the blaGES-11 ESBL gene and the blaOxA-23 CHDL gene. Analyses of the genetic location indicated that the blaGES-11 gene was plasmid located (Gr6). However, the blaOxA-23 gene was located on the chromosome. The second strain had only the blaOxA-23 CHDL gene, which was plasmid located. This study showed the first description of the GES-type β-lactamase in A. baumannii in Tunisia.
多重耐药且难以治疗的鲍曼不动杆菌可能是医院感染的病因。在全球范围内，越来越多的报道称鲍曼不动杆菌产生具有碳青霉烯酶活性的D类碳青霉烯水解β-内酰胺酶（CHDLs）和GES型超广谱β-内酰胺酶（ESBLs）。本研究的目的是分析在突尼斯中东部一家新生儿中心分离出的两株耐碳青霉烯鲍曼不动杆菌临床菌株的耐药机制。回收了两株耐碳青霉烯鲍曼不动杆菌菌株。第一株菌株同时携带blaGES-11 ESBL基因和blaOxA-23 CHDL基因。基因定位分析表明，blaGES-11基因位于质粒上（Gr6）。然而，blaOxA-23基因位于染色体上。第二株菌株仅含有位于质粒上的blaOxA-23 CHDL基因。本研究首次描述了突尼斯鲍曼不动杆菌中的GES型β-内酰胺酶。
