Al-Rashed Nouf, Bindayna Khalid M, Shahid Mohammad, Saeed Nermin Kamal, Darwish Abdullah, Joji Ronni Mol, Al-Mahmeed Ali
Department of Microbiology, Immunology, and Infectious Diseases, College of Medicine & Medical Sciences, Arabian Gulf University, Manama P.O. Box 26671, Bahrain.
Department of Pathology, Microbiology Section, Al- Salmaniya Medical Complex, Manama P.O. Box 12, Bahrain.
Antibiotics (Basel). 2023 Jul 17;12(7):1198. doi: 10.3390/antibiotics12071198.
is regarded as a significant cause of death in hospitals. The WHO recently added carbapenem-resistant (CRAB) to its global pathogen priority list. There is a dearth of information on CRAB from our region.
Fifty CRAB isolates were collected from four main hospitals in Bahrain for this study. Bacterial identification and antibiotic susceptibility tests were carried out using the BD Phoenix and VITEK-2 compact, respectively. Using conventional PCR, these isolates were further screened for carbapenem resistance markers ( and ).
All of the isolates were resistant to imipenem (100%), meropenem (98%), and cephalosporins (96-98%), followed by other commonly used antibiotics. All these isolates were least resistant to gentamicin (64%). The detection of resistance determinants showed that the majority harbored (100%) and (94%), followed by (82%), (46%), (14%), (6%), (2%), and (2%).
The study isolates showed a high level of antibiotic resistance. Class D carbapenemases were more prevalent in our CRAB isolate collection. The resistance genes were found in various combinations. This study emphasizes the importance of strengthening surveillance and stringent infection control measures in clinical settings to prevent the emergence and further spread of such isolates.
被视为医院内死亡的一个重要原因。世界卫生组织最近将耐碳青霉烯类鲍曼不动杆菌（CRAB）列入其全球病原体优先清单。我们所在地区关于CRAB的信息匮乏。
本研究从巴林的四家主要医院收集了50株CRAB分离株。分别使用BD Phoenix和VITEK-2 compact进行细菌鉴定和抗生素敏感性试验。通过常规PCR，对这些分离株进一步筛查碳青霉烯类耐药标志物（和）。
所有分离株均对亚胺培南（100%）、美罗培南（98%）和头孢菌素（96 - 98%）耐药，其次是其他常用抗生素。所有这些分离株对庆大霉素的耐药性最低（64%）。耐药决定因素的检测表明，大多数分离株携带（100%）和（94%），其次是（82%）、（46%）、（14%）、（6%）、（2%）和（2%）。
研究分离株显示出高水平的抗生素耐药性。D类碳青霉烯酶在我们收集的CRAB分离株中更为普遍。耐药基因以各种组合形式存在。本研究强调了在临床环境中加强监测和严格感染控制措施以预防此类分离株的出现和进一步传播的重要性。
