Jung Byeong Yeal, Seo Min-Goo, Lee Seung-Hun, Byun Jae-Won, Oem Jae-Ku, Kwak Dongmi
Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Anyang, South Korea.
College of Veterinary Medicine and Cardiovascular Research Institute, Kyungpook National University, Daegu, South Korea; Foot and Mouth Disease Division, Animal and Plant Quarantine Agency, Anyang, South Korea.
Vet Microbiol. 2014 Sep 17;173(1-2):152-5. doi: 10.1016/j.vetmic.2014.06.029. Epub 2014 Jul 7.
The occurrence of Q fever in native Korean goats (Capra hircus coreanae) was investigated for the first time in the country using ELISA and PCR. A total of 597 blood samples were collected from goats belonging to five different provinces of Korea. To detect Coxiella burnetii, sera were separated from the whole blood and analysed by ELISA; DNA was extracted directly from the whole blood and analysed by PCR. Overall, 114 (19.1%, 95% C.I.=16.1-22.4) and 57 goats (9.5%, 95% C.I.=7.5-12.2) tested positive for C. burnetii in the ELISA- and PCR-based screening, respectively, while 18 goats (3.0%, 95% C.I.=1.9-4.7) tested positive in both the assays. There was a significant difference between the number of ELISA- and PCR-positive goats (P<0.05). The seroprevalence of Q fever was significantly higher among the adult goats (≥1y, 22.0%) than among the young goats (<1y, 13.8%) (P<0.05). While the results of the serologic analysis showed no seasonal variation, data from the PCR-based assay indicated that there were a higher number of positive cases during the cold seasons. Because Q fever infection has high rates of prevalence in native Korean goats, further studies on humans at a high risk of contracting this disease should be conducted. The PCR-based assay used in this study is a useful method for the direct detection of C. burnetii in blood samples from small ruminants.
在韩国，首次使用酶联免疫吸附测定（ELISA）和聚合酶链反应（PCR）对韩国本土山羊（Capra hircus coreanae）进行Q热发生情况调查。从韩国五个不同省份的山羊身上共采集了597份血样。为检测伯氏考克斯体，从全血中分离血清并用ELISA进行分析；直接从全血中提取DNA并用PCR进行分析。总体而言，在基于ELISA和PCR的筛查中，分别有114只山羊（19.1%，95%置信区间=16.1 - 22.4）和57只山羊（9.5%，95%置信区间=7.5 - 12.2）检测出伯氏考克斯体呈阳性，而有18只山羊（3.0%，95%置信区间=1.9 - 4.7）在两种检测中均呈阳性。ELISA阳性山羊数量与PCR阳性山羊数量之间存在显著差异（P<0.05）。成年山羊（≥一岁，22.0%）的Q热血清阳性率显著高于幼年山羊（<一岁，13.8%）（P<0.05）。虽然血清学分析结果显示无季节性变化，但基于PCR检测的数据表明，寒冷季节阳性病例数量较多。由于Q热感染在韩国本土山羊中患病率较高，应进一步对感染该疾病风险较高的人群进行研究。本研究中使用的基于PCR的检测方法是直接检测小型反刍动物血样中伯氏考克斯体的有效方法。
