High prevalence of infection in humans and livestock in Assiut, Egypt: A serological and molecular survey.

BACKGROUND AND AIM

Q fever is considered a neglected zoonotic disease and is caused by . Very little information is available on infections in cattle, sheep, and goat populations in Egypt. The aim of this study was to identify the seroprevalence of in humans and livestock and to test for the presence of DNA in sera from seropositive animals and humans.

MATERIALS AND METHODS

Blood samples were collected from 160 apparently healthy farm animals and 120 patients from three hospitals of the Assiut Governorate throughout 2017/2018. These populations were tested for antibodies against phase II antigen by immunofluorescence assay [IFA] and enzyme-linked immunosorbent assay (ELISA). Seropositive samples were subjected to real-time quantitative polymerase chain reaction (RT-qPCR).

RESULTS

The results of the IFA revealed seroprevalence rates of 45.3%, 56.0%, 45.7%, and 53.3% in cattle, sheep, goats, and humans, respectively. In humans, the seroprevalence rates were 52.1%, 30.4%, 37.5%, 74.1%, and 62.5% in patients with fever of unknown origin, influenza, kidney dialysis, hepatitis C virus, and hepatitis B virus, respectively. Likewise, by ELISA, the seroprevalence in bovine was 50.7%; sheep, 60.0%; goats, 51.4%; and humans, 55.0% (54.3%, 30.4%, 37.5%, 77.8%, and 62.5% in patients with fever of unknown origin, influenza, kidney dialysis, hepatitis C virus, and hepatitis B virus, respectively). RT-qPCR targeting the repetitive element IS1111 confirmed the presence of DNA.

CONCLUSION

These results proved that apparently healthy cattle, sheep, and goats may be very important reservoirs of infection. In light of these data, the effect of Q fever on the replication of hepatitis virus remains unclear. Although hepatitis is one of the main aspects of acute Q fever, the influence of hepatitis on Q fever remains to be investigated. Q fever is not a reportable disease in Egypt, and clinical cases may rarely be recognized by the health-care system. Additional information on the epidemiology of in Egypt is warranted, including other associated problems such as the distribution of infections, pathologic hallmarks, and molecular typing.