Establishment of a new murine elastase-induced aneurysm model combined with transplantation.
作者信息
Rowinska Zuzanna, Gorressen Simone, Merx Marc W, Koeppel Thomas A, Liehn Elisa A, Zernecke Alma
机构信息
Department of Vascular and Endovascular Surgery, Düsseldorf University Hospital, Düsseldorf, Germany; Institute of Molecular Cardiovascular Research, University Hospital, RWTH Aachen University Department of Medicine, Aachen, Germany.
Division of Cardiology, Pneumology and Angiology, Düsseldorf University Hospital, Düsseldorf, Germany.
出版信息
PLoS One. 2014 Jul 28;9(7):e102648. doi: 10.1371/journal.pone.0102648. eCollection 2014.
INTRODUCTION
The aim of our study was to develop a reproducible murine model of elastase-induced aneurysm formation combined with aortic transplantation.
METHODS
Adult male mice (n = 6-9 per group) underwent infrarenal, orthotopic transplantation of the aorta treated with elastase or left untreated. Subsequently, both groups of mice were monitored by ultrasound until 7 weeks after grafting.
RESULTS
Mice receiving an elastase-pretreated aorta developed aneurysms and exhibited a significantly increased diastolic vessel diameter compared to control grafted mice at 7 week after surgery (1.11 ± 0.10 mm vs. 0.75 ± 0.03 mm; p ≤ 0,001). Histopathological examination revealed disruption of medial elastin, an increase in collagen content and smooth muscle cells, and neointima formation in aneurysm grafts.
CONCLUSIONS
We developed a reproducible murine model of elastase-induced aneurysm combined with aortic transplantation. This model may be suitable to investigate aneurysm-specific inflammatory processes and for use in gene-targeted animals.
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