Nasiri R, Hirbod-Mobarakeh A, Movahedi M, Farhadi E, Ansaripour B, Amirzargar A A, Rezaei N
Department of Pediatrics, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Universal Scientific Education and Research Network (USERN), Tehran, Iran; Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Students' Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Allergol Immunopathol (Madr). 2016 Mar-Apr;44(2):125-30. doi: 10.1016/j.aller.2015.05.010. Epub 2015 Aug 24.
Allergic rhinitis (AR) is a polygenic inflammatory disorder of the upper respiratory airway with an increasing prevalence worldwide. Interleukin-10 (IL-10) and transforming growth factor-beta (TGF-β), as two cytokines with pleiotropic effects on both innate and adaptive immunity, play important roles in allergic responses. Therefore, this study was performed to evaluate the associations of five polymorphisms of IL-10 and TGF-β genes with AR.
Ninety-eight patients with AR along with 140 healthy volunteers with no history of AR and with the same ethnicity of the patients were recruited in this study. Genotyping was done for three polymorphisms in promoter region of IL-10 gene (rs1800896, rs1800871, rs1800872), and two polymorphisms in the exonic region of TGF-β1 gene (rs1982037, rs1800471) using PCR sequence-specific-primers method.
A allele and AA genotype in rs1800896 of IL-10 and TT genotype in rs1982037 in TGF-β were significantly less frequent in the patients than in controls. While the C allele and the CG genotype in rs1800471 in TGF-β1 were associated with a higher susceptibility to AR. C/C and T/C haplotypes (rs1982037, rs1800471) in TGF-β1 gene and A/C/A, A/T/C and G/C/A haplotypes (rs1800896, rs1800871, rs1800872) in IL-10 gene were found with higher frequencies in patients than controls. Patients with CC genotype in rs1800871 in Il-10 had significantly lower levels of IgE.
We found that certain genetic variants in IL-10 and TGF-β polymorphisms were associated with susceptibility to AR as well as some clinical parameters in the patients with AR.
变应性鼻炎（AR）是一种上呼吸道的多基因炎症性疾病，在全球范围内患病率呈上升趋势。白细胞介素-10（IL-10）和转化生长因子-β（TGF-β）作为对固有免疫和适应性免疫均具有多效性作用的两种细胞因子，在变应性反应中发挥重要作用。因此，本研究旨在评估IL-10和TGF-β基因的5种多态性与AR的相关性。
本研究招募了98例AR患者以及140名无AR病史且与患者种族相同的健康志愿者。采用PCR序列特异性引物法对IL-10基因启动子区域的3种多态性（rs1800896、rs1800871、rs1800872）以及TGF-β1基因外显子区域的2种多态性（rs1982037、rs1800471）进行基因分型。
IL-10基因rs1800896的A等位基因和AA基因型以及TGF-β基因rs1982037的TT基因型在患者中的频率显著低于对照组。而TGF-β1基因rs1800471的C等位基因和CG基因型与AR的易感性较高相关。TGF-β1基因的C/C和T/C单倍型（rs1982037、rs1800471）以及IL-10基因的A/C/A、A/T/C和G/C/A单倍型（rs1800896、rs1800871、rs1800872）在患者中的频率高于对照组。IL-10基因rs1800871的CC基因型患者的IgE水平显著较低。
我们发现IL-10和TGF-β多态性中的某些基因变异与AR的易感性以及AR患者的一些临床参数相关。
