牛脂肪来源间充质干细胞（ADSCs）的分离与鉴定
Isolation and characterization of adipose-derived mesenchymal stem cells (ADSCs) from cattle.
作者信息
Lu Taofeng, Xiong Hui, Wang Kunfu, Wang Shuo, Ma Yuehui, Guan Weijun
机构信息
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
出版信息
Appl Biochem Biotechnol. 2014 Sep;174(2):719-28. doi: 10.1007/s12010-014-1128-3. Epub 2014 Aug 3.
Adipose-derived mesenchymal stem cells (ADSCs) were isolated from the adult adipose tissue of 2-year-old cattle, and then characterized by immunofluorescence and RT-PCR. We found that primary bADSCs could be expanded for 25 passages. Expression of β-integrin, CD44, and CD73 was observed by immunofluorescence and RT-PCR. Passage 3 bADSCs were successfully induced to differentiate into osteoblasts and adipocytes. The results indicate the potential for multi-lineage differentiation of bADSCs that may represent an ideal candidate for cellular transplantation therapy.
从2岁牛的成年脂肪组织中分离出脂肪间充质干细胞（ADSCs），然后通过免疫荧光和逆转录-聚合酶链反应（RT-PCR）对其进行表征。我们发现原代牛脂肪间充质干细胞（bADSCs）可传代培养25次。通过免疫荧光和RT-PCR观察到β-整合素、CD44和CD73的表达。第3代bADSCs成功诱导分化为成骨细胞和脂肪细胞。结果表明bADSCs具有多向分化潜能，可能是细胞移植治疗的理想候选者。
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