Lu Taofeng, Hu Pengfei, Su Xiaohua, Li Changli, Ma Yuehui, Guan Weijun
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Cell Tissue Bank. 2014 Sep;15(3):439-50. doi: 10.1007/s10561-013-9410-0. Epub 2013 Nov 28.
Bovine liver-derived mesenchymal stem cells (bLMSCs) were isolated from the liver tissue of 4-6 months old fetal calf, and then characterized by immunofluorescence and RT-PCR. We found that primary bLMSCs could be subcultured to 44 passages, the total culture time in vitro was 192 days. The results of surface antigen detection showed that bBMSCs expressed CD29, CD44, CD73, CD90, CD106 and CD166 but not expressed endothelial cells and hematopoietic cells specific marker CD34, CD45 and BLA-DR. The results of growth kinetics, colony-forming cell assay and cell cycle analysis indicated that the fetal bovine LMSCs had good proliferation ability in vitro. The cells from passages 7 were successfully induced to differentiate into osteoblasts, adipocytes and chondrocytes. The results indicate the potential for multi-lineage differentiation of bLMSCs that may represent an ideal candidate for cellular transplantation therapy.
牛肝脏来源的间充质干细胞（bLMSCs）从4至6月龄胎牛的肝脏组织中分离得到，然后通过免疫荧光和逆转录-聚合酶链反应（RT-PCR）进行鉴定。我们发现原代bLMSCs可传代培养至44代，体外总培养时间为192天。表面抗原检测结果显示，bBMSCs表达CD29、CD44、CD73、CD90、CD106和CD166，但不表达内皮细胞和造血细胞特异性标志物CD34、CD45和BLA-DR。生长动力学、集落形成细胞测定和细胞周期分析结果表明，胎牛LMSCs在体外具有良好的增殖能力。第7代细胞成功诱导分化为成骨细胞、脂肪细胞和软骨细胞。结果表明bLMSCs具有多向分化潜能，可能是细胞移植治疗的理想候选者。
