从（CCC540）中提取和纯化C-藻蓝蛋白。
Extraction and purification of C-phycocyanin from (CCC540).
作者信息
Kumar Devendra, Dhar Dolly Wattal, Pabbi Sunil, Kumar Neeraj, Walia Suresh
机构信息
Centre for Conservation and Utilisation of Blue Green Algae, Indian Agricultural Research Institute, New Delhi, 110012 India.
Microbiology Department, Kurukshetra University, Kurukshetra, India.
出版信息
Indian J Plant Physiol. 2014;19(2):184-188. doi: 10.1007/s40502-014-0094-7. Epub 2014 Jul 16.
In this study a simple protocol was developed for purifying phycocyanin (PC) from (CCC540) by using ammonium sulphate precipitation, followed by a single step chromatography by using DEAE-Cellulose-11 and acetate buffer. Precipitation with 65 % ammonium sulphate resulted in 80 % recovery of phycocyanin with purity of 1.5 (A/A). Thro1ugh chromatography an 80 % recovery of phycocyanin with a purity of 4.5 (A/A) was achieved. In SDS_PAGE analysis, the purified PC showed the presence of two subunit α (16 kD) and β (17 kD).
在本研究中，开发了一种简单的方案，通过硫酸铵沉淀从（CCC540）中纯化藻蓝蛋白（PC），随后使用DEAE-纤维素-11和乙酸盐缓冲液进行一步色谱法。用65%硫酸铵沉淀可使藻蓝蛋白回收率达80%，纯度为1.5（A/A）。通过色谱法可实现藻蓝蛋白80%的回收率，纯度为4.5（A/A）。在SDS_PAGE分析中，纯化的PC显示存在两个亚基α（16kD）和β（17kD）。
Zotero 插件