Haddad Nabila, Matos Rute G, Pinto Teresa, Rannou Pauline, Cappelier Jean-Michel, Prévost Hervé, Arraiano Cecília M
From the LUNAM Université, Oniris, University of Nantes, 44200 Nantes, France, the UMR1014 Sécurité des Aliments et Microbiologie, INRA, 44322 Nantes, France, and
the Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av da República, 2780-157 Oeiras, Portugal.
J Biol Chem. 2014 Oct 3;289(40):27814-24. doi: 10.1074/jbc.M114.561795. Epub 2014 Aug 6.
Bacterial pathogens must adapt/respond rapidly to changing environmental conditions. Ribonucleases (RNases) can be crucial factors contributing to the fast adaptation of RNA levels to different environmental demands. It has been demonstrated that the exoribonuclease polynucleotide phosphorylase (PNPase) facilitates survival of Campylobacter jejuni in low temperatures and favors swimming, chick colonization, and cell adhesion/invasion. However, little is known about the mechanism of action of other ribonucleases in this microorganism. Members of the RNB family of enzymes have been shown to be involved in virulence of several pathogens. We have searched C. jejuni genome for homologues and found one candidate that displayed properties more similar to RNase R (Cj-RNR). We show here that Cj-RNR is important for the first steps of infection, the adhesion and invasion of C. jejuni to eukaryotic cells. Moreover, Cj-RNR proved to be active in a wide range of conditions. The results obtained lead us to conclude that Cj-RNR has an important role in the biology of this foodborne pathogen.
细菌病原体必须迅速适应/应对不断变化的环境条件。核糖核酸酶（RNases）可能是促使RNA水平快速适应不同环境需求的关键因素。已证明外切核糖核酸酶多核苷酸磷酸化酶（PNPase）有助于空肠弯曲菌在低温下存活，并有利于其游动、在鸡体内定殖以及细胞黏附/侵袭。然而，对于这种微生物中其他核糖核酸酶的作用机制知之甚少。RNB酶家族的成员已被证明与几种病原体的毒力有关。我们在空肠弯曲菌基因组中搜索了同源物，发现了一个与核糖核酸酶R（Cj-RNR）表现出更相似特性的候选物。我们在此表明，Cj-RNR对于感染的第一步，即空肠弯曲菌对真核细胞的黏附和侵袭很重要。此外，Cj-RNR在广泛的条件下都具有活性。所获得的结果使我们得出结论，Cj-RNR在这种食源性病原体的生物学特性中具有重要作用。
