Christy B, Nathans D
Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Proc Natl Acad Sci U S A. 1989 Nov;86(22):8737-41. doi: 10.1073/pnas.86.22.8737.
Zif268, a zinc finger protein whose mRNA is rapidly activated in cells exposed to growth factors or other signaling agents, is thought to play a role in regulating the genetic program induced by extracellular ligands. We report that Zif268 has one of the characteristics of a transcriptional regulator, namely, sequence-specific binding to DNA. Zif268 synthesized in Escherichia coli bound to two sites upstream of the zif268 gene and to sites in the promoter regions of other genes. The nucleotide sequences responsible for binding were defined by DNase I footprinting, by methylation interference experiments, and by use of synthetic oligonucleotides. From these results we derived the following consensus sequence for a Zif268 high-affinity binding site: GCGTGGGGCG.
Zif268是一种锌指蛋白,其mRNA在暴露于生长因子或其他信号传导剂的细胞中会迅速被激活,被认为在调节由细胞外配体诱导的遗传程序中发挥作用。我们报告称,Zif268具有转录调节因子的一个特征,即与DNA的序列特异性结合。在大肠杆菌中合成的Zif268与zif268基因上游的两个位点以及其他基因启动子区域的位点结合。通过DNA酶I足迹法、甲基化干扰实验以及使用合成寡核苷酸确定了负责结合的核苷酸序列。从这些结果中,我们得出了Zif268高亲和力结合位点的以下共有序列:GCGTGGGGCG。