Searle P F, Davison B L, Stuart G W, Wilkie T M, Norstedt G, Palmiter R D
Mol Cell Biol. 1984 Jul;4(7):1221-30. doi: 10.1128/mcb.4.7.1221-1230.1984.
The mouse metallothionein II (MT-II) gene is located approximately 6 kilobases upstream of the MT-I gene. A comparison of the sequences of mouse MT-I and MT-II genes (as well as those of other mammals) reveals that the coding regions are highly conserved even at "silent" positions but that the noncoding regions and introns are extremely divergent between primates and rodents. There are four blocks of conserved sequences in the promoters of mouse MT-I, mouse MT-II, and human MT-IIA genes; one includes the TATAAA sequence, and another has been implicated in regulation by heavy metals. Mouse MT-I and MT-II mRNAs are induced to approximately the same extent in vivo in response to cadmium, dexamethasone, or lipopolysaccharide. Mouse MT-I and MT-II genes are regulated by metals but not by glucocorticoids after transfection into HeLa cells.
小鼠金属硫蛋白II（MT-II）基因位于MT-I基因上游约6千碱基处。对小鼠MT-I和MT-II基因（以及其他哺乳动物的基因）序列进行比较发现，即使在“沉默”位置，编码区也高度保守，但灵长类动物和啮齿动物之间的非编码区和内含子差异极大。小鼠MT-I、小鼠MT-II和人类MT-IIA基因的启动子中有四个保守序列块；一个包含TATAAA序列，另一个与重金属调节有关。在体内，镉、地塞米松或脂多糖可使小鼠MT-I和MT-II mRNA诱导至大致相同的程度。转染至HeLa细胞后，小鼠MT-I和MT-II基因受金属调节，但不受糖皮质激素调节。
