Michalik Anna, Jankowska Władysława, Kot Marta, Gołas Aniela, Szklarzewicz Teresa
Department of Developmental Biology and Morphology of Invertebrates, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387 Kraków, Poland.
Department of Genetics and Evolution, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387 Kraków, Poland.
Arthropod Struct Dev. 2014 Nov;43(6):579-87. doi: 10.1016/j.asd.2014.07.005. Epub 2014 Aug 5.
The green leafhopper, Cicadella viridis lives in symbiotic association with microorganisms. The ultrastructural and molecular analyses have shown that in the body of the C. viridis two types of bacteriocyte endosymbionts are present. An amplification and sequencing of 16S rRNA genes revealed that large, pleomorphic bacteria display a high similarity (94-100%) to the endosymbiont 'Candidatus Sulcia muelleri' (phylum Bacteroidetes), whereas long, rod-shaped microorganisms are closely related to the γ-proteobacterial symbiont Sodalis (97-99% similarity). Both endosymbionts may be harbored in their own bacteriocytes as well as may co-reside in the same bacteriocytes. The ultrastructural observations have revealed that the Sodalis-like bacteria harboring the same bacteriocytes as bacterium Sulcia may invade the cells of the latter. Bacteria Sulcia and Sodalis-like endosymbionts are transovarially transmitted from one generation to the next. However, Sodalis-like endosymbionts do not invade the ovaries individually, but only inside Sulcia cells. Apart from bacteriocyte endosymbionts, in the body of C. viridis small, rod-shaped bacteria have been detected, and have been identified as being closely related to γ-proteobacterial microorganism Pectobacterium (98-99% similarity). The latter are present in the sheath cells of the bacteriomes containing bacterium Sulcia as well as in fat body cells.
绿叶蝉（Cicadella viridis）与微生物共生。超微结构和分子分析表明，绿叶蝉体内存在两种类型的含菌细胞内共生体。对16S rRNA基因的扩增和测序显示，大型多形细菌与内共生体“Candidatus Sulcia muelleri”（拟杆菌门）具有高度相似性（94 - 100%），而长杆状微生物与γ-变形菌共生体 Sodalis 密切相关（相似性为97 - 99%）。两种内共生体既可以存在于各自的含菌细胞中，也可以共同存在于同一个含菌细胞中。超微结构观察表明，与 Sulcia 细菌存在于同一含菌细胞中的类 Sodalis 细菌可能会侵入后者的细胞。Sulcia 细菌和类 Sodalis 内共生体通过卵传递从一代传到下一代。然而，类 Sodalis 内共生体不会单独侵入卵巢，而是仅在 Sulcia 细胞内侵入。除了含菌细胞内共生体外，在绿叶蝉体内还检测到小型杆状细菌，并已确定它们与γ-变形菌微生物果胶杆菌密切相关（相似性为98 - 99%）。后者存在于含有 Sulcia 细菌的菌瘤鞘细胞以及脂肪体细胞中。
