Que Yan-Fu, Tang Hui-Yuan, Xu Nian, Xu Dong-Mei, Shi Fang, Yang Zhi, Li Wei-Tao
a Key Laboratory of Ecological Impacts of Hydraulic-Projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources , Institute of Hydroecology, Ministry of Water Resources and Chinese Academy of Sciences , Wuhan , P.R. China .
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):1297-9. doi: 10.3109/19401736.2014.945564. Epub 2014 Aug 8.
Homatula potanini is an endemic and one of ornamental fishes in the upper stream of the Yangtze River and its tributaries. However, wild populations of H. potanini declined sharply due to anthropological activity in the Jinsha River during the past decades. In present study, the complete mitochondrial DNA genome sequence of H. potanini was first determined by DNA sequencing based on the PCR fragments. The complete mitochondrial genome sequence is a circular molecule of 16,569 bp in size. It consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a control region (D-loop). The gene nucleotide composition of H. potanini is 30.1% A, 26.9% C, 16.7% G, and 26.3% T, with a relatively high A + T content (56.4%). The results could provide useful data for studies on genetic structure and rational resource conservation in H. potanini.
短须裂腹鱼是长江上游及其支流的特有鱼类和观赏鱼类之一。然而，在过去几十年里，由于金沙江的人类活动，短须裂腹鱼的野生种群数量急剧下降。在本研究中，首次通过基于PCR片段的DNA测序确定了短须裂腹鱼的完整线粒体DNA基因组序列。完整的线粒体基因组序列是一个大小为16569 bp的环状分子。它由13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和一个控制区（D-loop）组成。短须裂腹鱼的基因核苷酸组成为A占30.1%、C占26.9%、G占16.7%、T占26.3%，A+T含量相对较高（56.4%）。这些结果可为短须裂腹鱼的遗传结构研究和合理资源保护提供有用的数据。
