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澜沧江(硬骨鱼纲,鲤形目)的线粒体全基因组及其系统发育分析。

The complete mitochondrial genome of (Teleostei, Cypriniformes) in Lantsang River and its phylogenetic analysis.

作者信息

Li Rui, Yue Xingjian

机构信息

School of Life Science, Neijiang Normal University, Neijiang, China.

Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang, China.

出版信息

Mitochondrial DNA B Resour. 2020 Sep 29;5(3):3447-3449. doi: 10.1080/23802359.2020.1823901.

Abstract

is a small-sized benthopelagic fish species in the family Nemacheilidae, superfamily Cobitidea. In this study, the entire mitogenome sequence of the has been sequenced by using sanger sequencing method. The circular mitochondrial genome was 16,570 bp in length and contained of 13 protein-coding genes, two ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, an origin of light-strand replication (OL), and one displacement loop locus (D-loop). The overall base composition was 30.1% A, 26.4% T, 26.8% C, and 16.7% G with 56.6% AT content. Most genes were encoded on the heavy strand except for ND6 and eight tRNA genes. There were 11 regions of gene overlaps totaling 29 bp and 7 intergenic spacer regions totaling 37 bp. Its structure type was similar to the mitogenome of Cobitidea fishes. Phylogenetic analysis with 33 complete mitogenomes of Cobitidea and fishes in the family of Mastacembelidae suggested that species clustered as one monophyletic clade and was closely related to Mitogenome information from this study could be a useful basis for conservation and phylogenetics of this species.

摘要

是鳅科、鳅超科中的一种小型底栖性鱼类。在本研究中,已使用桑格测序法对其完整的线粒体基因组序列进行了测序。环状线粒体基因组长度为16,570 bp,包含13个蛋白质编码基因、两个核糖体RNA(rRNA)基因、22个转运RNA(tRNA)基因、一个轻链复制起点(OL)和一个位移环位点(D-loop)。总体碱基组成为30.1%A、26.4%T、26.8%C和16.7%G,AT含量为56.6%。除了ND6和8个tRNA基因外,大多数基因在重链上编码。有11个基因重叠区域,总计29 bp,7个基因间隔区,总计37 bp。其结构类型与鳅超科鱼类的线粒体基因组相似。对鳅超科的33个完整线粒体基因组和刺鳅科鱼类进行的系统发育分析表明,该物种聚为一个单系类群,且与[具体物种名称]密切相关。本研究的线粒体基因组信息可为该物种的保护和系统发育研究提供有用的基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68f4/7783039/e533fe6c6ca9/TMDN_A_1823901_F0001_C.jpg

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