Laboratory of Biomedical Optics and Imaging, Federal University of Pernambuco, Recife, Brazil.
Physics Department, Federal University of Paraíba, João Pessoa, Brazil.
Methods Mol Biol. 2020;2135:37-51. doi: 10.1007/978-1-0716-0463-2_2.
Optical spectroscopy techniques are crucial for the evaluation and use of quantum dots (QDs) in life and materials science. In that context, the fluorescence quantum yield (Φ) is an essential parameter in the assessment of the luminescent features of QDs. The fluorescence quantum yield can be defined as the ratio of the number of emitted photons to the number of absorbed photons by a luminescent material. In this chapter, we describe absolute and relative methods to measure the fluorescence quantum yield of QDs in solution phase. The advantages and limitations of the techniques are reviewed.
光学光谱技术对于评估和应用量子点(QD)在生命科学和材料科学领域至关重要。在这种情况下,荧光量子产率(Φ)是评估 QD 发光特性的一个重要参数。荧光量子产率可以定义为发光材料发射的光子数与吸收的光子数之比。本章描述了用于测量溶液中 QD 荧光量子产率的绝对和相对方法。并对这些技术的优缺点进行了综述。