Mesquita R P
Instituto Oswaldo Cruz, Department of Pathology, Rio de Janeiro, Brazil.
Virchows Arch A Pathol Anat Histopathol. 1989;416(1):1-9. doi: 10.1007/BF01606464.
A new and simple method to study the inflammatory process in vivo using a diffusion-migration chamber is described. The influx of inflammatory elements into chambers was studied by light and electron microscopy. This gradient chamber (GC) was developed by substituting one of the filters of the classic diffusion chamber by a polyvinyl sponge cylinder. In the implanted chambers this plastic framework acts as a tridimensional gradient, through which the acute and chronic inflammatory processes migrate with different speeds and are thus divided into two distinct and widely separated waves. By delaying the overlapping of the first wave by the second, this bioassay allows a longer time for observation of the cell-matrix or cell-matrix-graft interaction during the first inflammatory wave and permits studies by methods such as immunocytochemistry, histochemistry or biochemical analysis.
本文描述了一种使用扩散迁移室在体内研究炎症过程的新的简单方法。通过光学显微镜和电子显微镜研究炎症成分流入室中的情况。这种梯度室（GC）是通过用聚乙烯海绵圆柱体替代经典扩散室的一个滤膜而开发的。在植入的室中，这种塑料框架充当三维梯度，急性和慢性炎症过程以不同速度通过该梯度迁移，从而分为两个明显且相隔很远的波。通过延迟第二波与第一波的重叠，这种生物测定法可以在第一炎症波期间有更长的时间观察细胞-基质或细胞-基质-移植物相互作用，并允许通过免疫细胞化学、组织化学或生化分析等方法进行研究。
