Ruozi Barbara, Belletti Daniela, Forni Flavio, Sharma Aruna, Muresanu Dafin, Mössler Herbert, Vandelli Maria A, Tosi Giovanni, Sharma Hari S
Director of Research, International Experimental CNS Injury & Repair, Laboratory of Cerebrovascular Research, Department of Surgical Sciences, Anesthesiology & Intensive Care Medicine, University Hospital, Uppsala University, SE-75421, Uppsala, Sweden.
CNS Neurol Disord Drug Targets. 2014;13(8):1475-82. doi: 10.2174/1871527313666140806145540.
Cerebrolysin (CBL) is a neuroprotective agent in central nervous system (CNS) injury and stimulates neurorepair processes. Several studies in our laboratory suggest that CBL administered through nanowired technology may have superior neuroprotective efficacy in CNS trauma. In this investigation, we compared the neuroprotective efficacy of poly-lactide-co-glycolide nanoparticles (NPs) loaded with CBL vs free CBL in a rat model of concussive head injury (CHI). Free CBL or CBL loaded NPs was administered 30 min to 1 h after CHI and animals were sacrificed 5 h later. Changes in blood-brain barrier and brain edema formation were measured as parameters of neuroprotection in CHI after giving CBL alone or as the nanodelivered compound. Our results clearly show that delivery of CBL by NPs has superior neuroprotective effects following CHI as compared to normal CBL. This suggests that CBL delivered by NPs could have robust neuroprotective action in CNS trauma. These findings have potential clinical relevance with regard to nanodelivery of CBL, a feature that requires further investigation.
脑蛋白水解物（CBL）是一种用于中枢神经系统（CNS）损伤的神经保护剂，可刺激神经修复过程。我们实验室的多项研究表明，通过纳米线技术给药的CBL在中枢神经系统创伤中可能具有更好的神经保护效果。在本研究中，我们在大鼠脑震荡损伤（CHI）模型中比较了载有CBL的聚乳酸-羟基乙酸共聚物纳米颗粒（NPs）与游离CBL的神经保护效果。在CHI后30分钟至1小时给予游离CBL或载有CBL的NPs，5小时后处死动物。在单独给予CBL或作为纳米递送化合物后，测量血脑屏障的变化和脑水肿形成，作为CHI中神经保护的参数。我们的结果清楚地表明，与普通CBL相比，NPs递送的CBL在CHI后具有更好的神经保护作用。这表明NPs递送的CBL在中枢神经系统创伤中可能具有强大的神经保护作用。这些发现对于CBL的纳米递送具有潜在的临床意义，这一特性需要进一步研究。
