Tsubouchi Taishi, Koyama Sumihiro, Mori Kozue, Shimane Yasuhiro, Usui Keiko, Tokuda Maki, Tame Akihiro, Uematsu Katsuyuki, Maruyama Tadashi, Hatada Yuji
Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan.
Section1 Geochemical Oceanography, Office of Marine Research Department of Marine Science, Marine Works Japan Ltd, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan.
Int J Syst Evol Microbiol. 2014 Nov;64(Pt 11):3709-3716. doi: 10.1099/ijs.0.067199-0. Epub 2014 Aug 8.
A novel Gram-stain-negative, aerobic, heterotrophic, stalked and capsulated bacterium with potential denitrification ability, designated strain TAR-002(T), was isolated from deep seafloor sediment in Japan. Colonies lacked lustre, and were viscous and translucent white. The ranges of temperature, pH and salt concentration for growth were 8-30 °C, pH 6.0-10.0 and 1-3% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain TAR-002(T) belongs to the genus Brevundimonas of the class Alphaproteobacteria. Levels of similarity between the 16S rRNA gene sequence of strain TAR-002(T) and those of the type strains of species of the genus Brevundimonas were 93.5-98.9%; the most closely related species was Brevundimonas basaltis. In DNA-DNA hybridization assays between strain TAR-002(T) and its phylogenetic neighbours, Brevundimonas lenta DS-18(T), B. basaltis J22(T), Brevundimonas subvibrioides ATCC 15264(T) and Brevundimonas alba DSM 4736(T), mean hybridization levels were 6.4-27.7%. The G+C content of strain TAR-002(T) was 70.3 mol%. Q-10 was the major respiratory isoprenoid quinone. The major fatty acids were C(18:1)ω7c and C(16:0), and the presence of 1,2-di-O-acyl-3-O-[D-glucopyranosyl-(1 → 4)-α-D-glucopyranuronosyl]glycerol (DGL) indicates the affiliation of strain TAR-002(T) with the genus Brevundimonas. On the basis of biological characteristics and 16S rRNA gene sequence comparisons, strain TAR-002(T) is considered to represent a novel species of the genus Brevundimonas, for which the name Brevundimonas denitrificans sp. nov. is proposed; the type strain is TAR-002(T) ( =NBRC 110107(T) =CECT 8537(T)).
从日本深海海底沉积物中分离出一株具有潜在反硝化能力的新型革兰氏阴性、需氧、异养、有柄且有荚膜的细菌，命名为菌株TAR-002(T)。菌落无光泽，粘性且半透明呈白色。其生长的温度范围为8-30℃，pH值范围为6.0-10.0，盐浓度范围为1-3%（w/v）NaCl。基于16S rRNA基因序列的系统发育分析证实，菌株TAR-002(T)属于α-变形菌纲短波单胞菌属。菌株TAR-002(T)的16S rRNA基因序列与短波单胞菌属各物种模式菌株的序列相似性水平为93.5-98.9%；最密切相关的物种是玄武岩短波单胞菌。在菌株TAR-002(T)与其系统发育近缘种慢生短波单胞菌DS-18(T)、玄武岩短波单胞菌J22(T)、类弧状短波单胞菌ATCC 15264(T)和白色短波单胞菌DSM 4736(T)之间的DNA-DNA杂交试验中，平均杂交水平为6.4-27.7%。菌株TAR-002(T)的G+C含量为70.3 mol%。泛醌-10是主要的呼吸类异戊二烯醌。主要脂肪酸为C(18:1)ω7c和C(16:0)，1,2-二-O-酰基-3-O-[D-吡喃葡萄糖基-(1→4)-α-D-吡喃葡萄糖醛酸基]甘油（DGL）的存在表明菌株TAR-002(T)属于短波单胞菌属。基于生物学特性和16S rRNA基因序列比较，菌株TAR-002(T)被认为代表短波单胞菌属的一个新物种，为此提出名为反硝化短波单胞菌（Brevundimonas denitrificans sp. nov.）；模式菌株为TAR-002(T)（=NBRC 110107(T)=CECT 8537(T)）。
