Dahal Ram Hari, Kim Jaisoo
Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do 16227, Republic of Korea.
Int J Syst Evol Microbiol. 2018 Mar;68(3):709-714. doi: 10.1099/ijsem.0.002559. Epub 2018 Jan 15.
During a study of bacterial diversity of soil, a novel strain, CA-15, was isolated from Kyonggi University forest soil. Cells were aerobic, Gram-stain-negative, motile, non-spore-forming, rod-shaped, oxidase-positive and catalase- negative. Tyrosine was not oxidized but produced red pigmentation on an agar palte. Strain CA-15 hydrolysed Tween 60 and DNA. It grew at 15-35 °C (optimum, 25-30 °C), pH 6.0-10.0 (optimum, 7.0-9.0) and at 1.5 % (w/v) NaCl concentration. Phylogenetic analysis based on its 16S rRNA gene sequence indicated that strain CA-15 formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria that was distinct from various species of the genus Brevundimonas. Brevundimonas bullata DSM 7126 was the closest member of strain CA-15 on the basis of 16S rRNA gene sequence similarity (98.48 %). Q-10 was only an isoprenoid quinone detected for strain CA-15. The major polar lipids were 1,2-di-O-acyl-3-O-[d-glucopyranosyl-(1→4)-αd-glucopyranuronosyl]glycerol, 1,2-di-O-acyl-3-O-[αd-glucopyranosyl]-sn-glycerol, 1,2-di-O-acyl-3-O-αd-glucopyranuronosylglycerol, 1,2-diacyl-3-O-[6'-phosphatidyl-αd-glucopyranosyl]glycerol and phosphatidylglycerol. The major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), C16 : 0, C18 : 1ω7c 11-methyl and C17 : 1ω8c. The DNA G+C content of strain CA-15 was 63.6 mol%. The polyphasic characterization indicated that strain CA-15 represents a novel species in the genus Brevundimonas, for which the name Brevundimonas humi sp. nov. is proposed. The type strain of Brevundimonas humi is CA-15 (=KEMB 9005-528=KACC 19106=NBRC 112677).
在一项关于土壤细菌多样性的研究中，从韩国庆熙大学森林土壤中分离出了一株新菌株CA-15。该菌株细胞为需氧型，革兰氏染色阴性，具运动性，不产芽孢，杆状，氧化酶阳性，过氧化氢酶阴性。酪氨酸不被氧化，但在琼脂平板上产生红色色素。菌株CA-15能水解吐温60和DNA。它能在15 - 35 °C（最适温度为25 - 30 °C）、pH 6.0 - 10.0（最适pH为7.0 - 9.0）以及1.5 %（w/v）的NaCl浓度下生长。基于其16S rRNA基因序列的系统发育分析表明，菌株CA-15在α-变形菌纲柄杆菌科内形成了一个分支，与短波单胞菌属的各种物种不同。基于16S rRNA基因序列相似性（98.48 %），泡囊短波单胞菌DSM 7126是菌株CA-15最接近的成员。菌株CA-15仅检测到一种异戊二烯醌Q-10。主要极性脂类为1,2 - 二 - O - 酰基 - 3 - O - [d - 吡喃葡萄糖基 - (1→4) - α - d - 吡喃葡萄糖醛酸基]甘油、1,2 - 二 - O - 酰基 - 3 - O - [α - d - 吡喃葡萄糖基] - sn - 甘油、1,2 - 二 - O - 酰基 - 3 - O - α - d - 吡喃葡萄糖醛酸基甘油、1,2 - 二酰基 - 3 - O - [6'-磷脂酰 - α - d - 吡喃葡萄糖基]甘油和磷脂酰甘油。主要细胞脂肪酸为总和特征8（C18 : 1ω7c和/或C18 : 1ω6c）、C16 : 0、C18 : 1ω7c 11 - 甲基和C17 : 1ω8c。菌株CA-15的DNA G + C含量为63.6 mol%。多相分类特征表明，菌株CA-15代表了短波单胞菌属中的一个新物种，为此提出名为土壤短波单胞菌（Brevundimonas humi sp. nov.）。土壤短波单胞菌的模式菌株为CA-15（=KEMB 9005 - 528=KACC 19106=NBRC 112677）。
