Qu Jian-Hang, Fu Yun-Hui, Li Xiao-Dan, Li Hai-Feng, Tian Hai-Long
College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, Henan, Province, PR China.
Int J Syst Evol Microbiol. 2019 May;69(5):1417-1422. doi: 10.1099/ijsem.0.003330. Epub 2019 Mar 6.
A novel Gram-negative, aerobic, rod-shaped bacterium, designated NS26, was isolated from a sediment sample collected from Taihu Lake in China. Colonies were orange, circular, smooth and neat-edged on Reasoner's 2A agar. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NS26 belonged to the genus Brevundimonas and had the closest relationship with Brevundimonas halotolerans DSM 24448 (96.9 %). It grew at 20-37 °C (optimum, 28 °C), pH 5.5-10.5 (pH 7.0) and without NaCl. The major isoprenoid quinone was Q-10. The dominant cellular fatty acids were C18 : 1ω7c, C16 : 0 and C18 : 1ω7c 11-methyl. The polar lipid profile comprised 1,2-diacyl-3-O-(6-phosphatidyl-α-d-glucopyranosyl) glycerol, 1,2-di-O-acyl-3-O-α-d-glycopyranuronosyl glycerol, sulfoquinovosyl diacylglycerol, 1,2-di-O-acyl-3-O-[d-glycopyranosyl-(1→4)-α-d-glucopyranuronosyl] glycerol and phosphatidylglycerol. The G+C content of genomic DNA was 68.4 mol%. The average nucleotide identity value between strain NS26 and B.halotolerans DSM 24448 was 75.6 %. Based on the polyphasic taxonomic study, strain NS26 is suggested to be a novel species, for which the name Brevundimonas lutea sp. nov. is proposed. The type strain is NS26 (=CGMCC 1.13680=NBRC 113554).
从中国太湖采集的沉积物样本中分离出一株新型革兰氏阴性需氧杆菌，命名为NS26。该菌在R2A琼脂平板上的菌落呈橙色、圆形、光滑且边缘整齐。基于16S rRNA基因序列的系统发育分析表明，菌株NS26属于短波单胞菌属，与耐盐短波单胞菌DSM 24448亲缘关系最近（相似度为96.9%）。它在20-37 °C（最适温度28 °C）、pH 5.5-10.5（pH 7.0）且无氯化钠的条件下生长。主要类异戊二烯醌为Q-10。主要的细胞脂肪酸为C18 : 1ω7c、C16 : 0和C18 : 1ω7c 11-甲基。极性脂质谱包含1,2-二酰基-3-O-(6-磷脂酰-α-d-吡喃葡萄糖基)甘油、1,2-二-O-酰基-3-O-α-d-吡喃糖醛酸基甘油、磺基喹诺糖基二酰基甘油、1,2-二-O-酰基-3-O-[d-吡喃葡萄糖基-(1→4)-α-d-吡喃糖醛酸基]甘油和磷脂酰甘油。基因组DNA的G+C含量为68.4 mol%。菌株NS26与耐盐短波单胞菌DSM 24448之间的平均核苷酸同一性值为75.6%。基于多相分类研究，建议将菌株NS26认定为一个新物种，命名为黄色短波单胞菌（Brevundimonas lutea sp. nov.）。模式菌株为NS26（=CGMCC 1.13680=NBRC 113554）。
