Mozo L, López P, Caminal-Montero L, Rodríguez-Carrio J, Suárez A
Department of Immunology, Hospital Universitario Central de Asturias, Oviedo, Spain
Department of Functional Biology, Immunology Area, Universidad de Oviedo, Oviedo, Spain.
Lupus. 2014 Dec;23(14):1477-85. doi: 10.1177/0961203314546020. Epub 2014 Aug 8.
The objective of this paper is to analyze the relationship of anti-protein ribosomal P (RibP) antibodies with circulating levels of IFNα, TNFα, IFNγ, IL-17 and IL-10 in SLE. Disease activity and other systemic lupus erythematosus (SLE) features were also analyzed.
Anti-RibP and other SLE-related antinuclear antibodies (ANA) were determined by fluoro-enzyme immunoassay in the sera of 107 SLE patients. Circulating cytokines were quantified by flow cytometry (IFNα, IL-10 and IL-17) or ELISA (TNFα and IFNγ).
Anti-RibP-positive patients (14.9%) displayed significantly higher serum levels of IFNα (p = 0.023) and IL-10 (p = 0.016) than their negative counterparts. This cytokine upregulation was independent of the presence of other ANA even though, in our patient cohort, anti-dsDNA was found to be associated with anti-RibP (OR, CI 95%: 6.03, 1.32-27.93, p = 0.021) and to correlate with IL-10 levels (r = 0.204, p = 0.036). In fact, patients positive for anti-RibP but negative for anti-dsDNA exhibited the highest amounts of both IL-10 and IFN-α that were not related to disease activity since these patients showed lower SLEDAI than patients also positive for anti-dsDNA (p = 0.018). Anti-RibP positivity was also associated with early diagnosis, hypocomplementemia and leukopenia.
Presence of anti-RibP was found to be related to increased serum IFNα and IL-10 levels independently of both antibody status and disease activity.
本文旨在分析抗核糖体P蛋白（RibP）抗体与系统性红斑狼疮（SLE）患者循环中干扰素α（IFNα）、肿瘤坏死因子α（TNFα）、干扰素γ（IFNγ）、白细胞介素17（IL - 17）和白细胞介素10（IL - 10）水平之间的关系。同时还分析了疾病活动度及其他系统性红斑狼疮（SLE）特征。
采用荧光酶免疫分析法检测107例SLE患者血清中的抗RibP及其他与SLE相关的抗核抗体（ANA）。通过流式细胞术（检测IFNα、IL - 10和IL - 17）或酶联免疫吸附测定（ELISA，检测TNFα和IFNγ）对循环细胞因子进行定量分析。
抗RibP阳性患者（14.9%）血清中IFNα（p = 0.023）和IL - 10（p = 0.016）水平显著高于抗RibP阴性患者。即使在我们的患者队列中发现抗双链DNA（dsDNA）与抗RibP相关（比值比，95%置信区间：6.03，1.32 - 27.93，p = 0.021）且与IL - 10水平相关（r = 0.204，p = 0.036），这种细胞因子上调也与其他ANA的存在无关。实际上，抗RibP阳性但抗dsDNA阴性的患者IL - 10和IFN - α水平最高，且与疾病活动度无关，因为这些患者的系统性红斑狼疮疾病活动指数（SLEDAI）低于抗dsDNA也阳性的患者（p = 0.018）。抗RibP阳性还与早期诊断、低补体血症和白细胞减少有关。
发现抗RibP的存在与血清IFNα和IL - 10水平升高有关，且与抗体状态和疾病活动度均无关。
