Adam Nathalie, Leroux Frédéric, Knapen Dries, Bals Sara, Blust Ronny
Systemic Physiological and Ecotoxicological Research, Department of Biology, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerpen, Belgium.
Electron Microscopy for Materials Science (EMAT), Department of Physics, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerpen, Belgium.
Environ Pollut. 2014 Nov;194:130-137. doi: 10.1016/j.envpol.2014.06.037. Epub 2014 Aug 7.
In this study the uptake of ZnO and CuO nanoparticles by Daphnia magna was tested. Daphnids were exposed during 48 h to acute concentrations of the nanoparticles and corresponding metal salts. The Daphnia zinc and copper concentration was measured and the nanoparticles were localized using electron microscopy. The aggregation and dissolution in the medium was characterized. A fast dissolution of ZnO in the medium was observed, while most CuO formed large aggregates and only a small fraction dissolved. The Daphnia zinc concentration was comparable for the nanoparticles and salts. Contrarily, a much higher Daphnia copper concentration was observed in the CuO exposure, compared to the copper salt. CuO nanoparticles adsorbed onto the carapace and occurred in the gut but did not internalize in the tissues. The combined dissolution and uptake results indicate that the toxicity of both nanoparticle types was caused by metal ions dissolved from the particles in the medium.
在本研究中，测试了大型溞对氧化锌和氧化铜纳米颗粒的摄取情况。将溞暴露于纳米颗粒和相应金属盐的急性浓度下48小时。测量了溞体内锌和铜的浓度，并使用电子显微镜对纳米颗粒进行定位。对介质中的聚集和溶解情况进行了表征。观察到氧化锌在介质中快速溶解，而大多数氧化铜形成大聚集体，只有一小部分溶解。纳米颗粒和盐类的溞体锌浓度相当。相反，与铜盐相比，在氧化铜暴露组中观察到溞体铜浓度要高得多。氧化铜纳米颗粒吸附在甲壳上并出现在肠道中，但未内化到组织中。溶解和摄取的综合结果表明，两种类型纳米颗粒的毒性都是由介质中颗粒溶解出的金属离子引起的。
