Payre F, Yanicostas C, Vincent A
Centre de Recherches de Biologie et Génétique Cellulaires du CNRS, Toulouse, France.
Dev Biol. 1989 Dec;136(2):469-80. doi: 10.1016/0012-1606(89)90272-8.
Serendipity delta (sry delta) is a member of a set of Drosophila zinc finger protein genes showing maximal transcription during oogenesis. By using transformant lines, we monitored the zygotic expression of the sry delta gene and characterized some biochemical properties of a sry delta/beta-galactosidase fusion protein-containing fingers. Further analysis made use of anti-sry delta specific antibodies. During oogenesis, while sry delta mRNAs transcribed by nurse cells are transferred to the oocyte starting in stage 10, translation into protein occurs in the ooplasm starting in stage 12. The maternally inherited protein concentrates in embryonic nuclei during early cleavages, prior to the onset of zygotic transcription. At the blastoderm stage, the sry delta protein is localized in all somatic nuclei. Later in embryogenesis and up to the adult stage, the zygotic protein is present in nuclei of transcriptionally active cells (both somatic and germ line). These data are consistent with the sry delta protein being a transcription factor, with a role in zygotic activation of general cellular functions.
意外发现δ（sryδ）是一组果蝇锌指蛋白基因的成员，在卵子发生过程中表现出最大转录水平。通过使用转化株系，我们监测了sryδ基因的合子表达，并对含sryδ/β-半乳糖苷酶融合蛋白的锌指的一些生化特性进行了表征。进一步的分析利用了抗sryδ特异性抗体。在卵子发生过程中，虽然由滋养细胞转录的sryδ mRNA从第10阶段开始转移到卵母细胞中，但蛋白质的翻译从第12阶段开始在卵质中发生。母系遗传的蛋白质在合子转录开始之前的早期卵裂过程中集中在胚胎细胞核中。在囊胚期，sryδ蛋白定位于所有体细胞核中。在胚胎发育后期直至成虫阶段，合子蛋白存在于转录活跃细胞（体细胞和生殖细胞系）的细胞核中。这些数据与sryδ蛋白作为一种转录因子一致，在合子激活一般细胞功能中发挥作用。
