内脏利什曼病：采用间接荧光抗体技术对来自阿扎德查谟和克什米尔流行区的289名儿童进行的血清流行病学研究。

Visceral leishmaniasis: a seroepidemiological study of 289 children from endemic foci in Azad Jammu and Kashmir by indirect fluorescent antibody technique.

作者信息

Rab M A, Iqbal J, Azmi F H, Munir M A, Saleem M

出版信息

J Pak Med Assoc. 1989 Sep;39(9):225-8.

Abstract

Between January '85 and August '87, 22 cases of VL were seen at National Institute of Health, Islamabad. Three (14.6%) came from the previously known endemic region of Gilgit, 15 (68.1%) from different localities in Azad Jammu and Kashmir (AJ&K), and 4 (17.3%) from neighbouring foci in NWFP and Punjab. Mean age of the patients was 4.2 years, (Range, 10 months to 57 years) median 2.5 years and mode 2 years. High levels of Leishmania antibodies were detected by Indirect Immunofluorescent Antibody Technique (IFAT) in all cases. Leishmania were isolated from bone marrow aspirates of 2 patients and isoenzyme characterization performed in one of these, the organism was typed as Leishmania infantum sensu stricto. Sera from 289 children residing in 5 endemic localities in AJ&K was tested for Leishmania specific antibodies by IFAT and low levels of these antibodies were detected in 15.4% of the cases.

摘要

1985年1月至1987年8月期间，伊斯兰堡国立卫生研究院共收治了22例内脏利什曼病患者。其中3例（14.6%）来自此前已知的吉尔吉特流行地区，15例（68.1%）来自阿扎德克什米尔（AJ&K）的不同地区，4例（17.3%）来自西北边境省和旁遮普邦的邻近疫源地。患者的平均年龄为4.2岁（范围为10个月至57岁），中位数为2.5岁，众数为2岁。所有病例均通过间接免疫荧光抗体技术（IFAT）检测到高水平的利什曼原虫抗体。从2例患者的骨髓穿刺物中分离出利什曼原虫，并对其中1例进行了同工酶鉴定，该病原体被鉴定为狭义婴儿利什曼原虫。通过IFAT对居住在AJ&K 5个流行地区的289名儿童的血清进行了利什曼原虫特异性抗体检测，15.4%的病例中检测到低水平的这些抗体。