Motawi Tarek M K, Shaker Olfat G, El-Sawalhi Maha M, Abdel-Nasser Zeinab M
a Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Genome. 2014 May;57(5):259-66. doi: 10.1139/gen-2014-0022. Epub 2014 Jul 23.
Diabetes mellitus is one of the main threats to human health in the 21st century. Visfatin/Nampt and resistin are novel adipokines that have been implicated in the pathogenesis of type 2 diabetes mellitus (T2DM) and cardiovascular disease (CVD) complication. Several genetic studies have shown inconsistent results regarding association of visfatin/Nampt gene (NAMPT) and resistin gene (RETN) polymorphisms with T2DM and CVD complications. Here, we investigate whether NAMPT -948G/T and RETN -420C/G polymorphisms are associated with T2DM, its CVD complications, and serum adipokines levels in 90 Egyptian diabetic patients (44 without CVD and 46 with CVD) along with 60 healthy control subjects. Higher frequencies of NAMPT -948G/G and RETN -420G/G were observed among T2DM patients compared with controls. Furthermore, the frequencies of these genotypes were significantly higher in T2DM patients with CVD than those without CVD. Both NAMPT -948G/G and RETN -420G/G genotypes and G alleles were significantly associated with T2DM and CVD in Egyptian diabetic patients. Moreover, serum visfatin/Nampt and resistin levels were markedly elevated in T2DM patients, with the highest values observed in G/G genotypes among T2DM patients with CVD. In addition, positive correlations were observed between plasma adipokines levels and CVD risk factors. In conclusion, our data suggests that genetic variations in NAMPT -948G/T and RETN -420C/G may contribute to the disposition for T2DM and its CVD complications in Egyptian patients. However, further studies with greater sample size should be performed to verify these results.
糖尿病是21世纪对人类健康的主要威胁之一。内脂素/烟酰胺磷酸核糖转移酶（Visfatin/Nampt）和抵抗素是新型脂肪因子，与2型糖尿病（T2DM）和心血管疾病（CVD）并发症的发病机制有关。几项基因研究显示，关于内脂素/烟酰胺磷酸核糖转移酶基因（NAMPT）和抵抗素基因（RETN）多态性与T2DM和CVD并发症之间的关联，结果并不一致。在此，我们调查了90名埃及糖尿病患者（44名无CVD，46名有CVD）以及60名健康对照者中，NAMPT -948G/T和RETN -420C/G多态性是否与T2DM、其CVD并发症及血清脂肪因子水平相关。与对照组相比，T2DM患者中观察到更高频率的NAMPT -948G/G和RETN -420G/G。此外，这些基因型的频率在有CVD的T2DM患者中显著高于无CVD的患者。在埃及糖尿病患者中，NAMPT -948G/G和RETN -420G/G基因型及G等位基因均与T2DM和CVD显著相关。此外，T2DM患者血清内脂素/烟酰胺磷酸核糖转移酶和抵抗素水平显著升高，在有CVD的T2DM患者中，G/G基因型者的值最高。此外，血浆脂肪因子水平与CVD危险因素之间存在正相关。总之，我们的数据表明，NAMPT -948G/T和RETN -420C/G的基因变异可能促成埃及患者发生T2DM及其CVD并发症的倾向。然而，应进行更大样本量的进一步研究以验证这些结果。
