Hu Guanghui, Lai Peng, Liu Min, Xu Liang, Guo Zhuifeng, Liu Huan, Li Wei, Wang Gangchun, Yao Xudong, Zheng Junhua, Xu Yunfei
Department of Urology, Shanghai Tenth People's Hospital, Tongji University, 301 Yanchang Road, Shanghai, 200072, China,
Tumour Biol. 2014 Nov;35(11):11443-53. doi: 10.1007/s13277-014-2476-x. Epub 2014 Aug 15.
MicroRNAs play a crucial role in cancer progression and metastasis. miR-203a has been identified as a tumor suppressor in various cancers. However, its functions in renal cell carcinoma have not been illustrated. In this study, we detected the miR-203a expression in renal cell carcinoma and evaluated its association with clinical features. Overexpression of miR-203a was found in renal cell carcinoma tissues and renal cell carcinoma cells. High miR-203a expression is correlated with tumor stage and short overall survival time. Bioinformatics and luciferase assay confirmed that glycogen synthase kinase-3β was a target gene of miR-203a. Silencing of miR-203a could inhibit cell proliferation and migration, arrest them in G1 phase, and promote apoptosis in vitro. miR-203a promotes the progression of renal cell carcinoma and predicts a poor prognosis.
微小RNA在癌症进展和转移中发挥着关键作用。miR-203a已被确定为多种癌症中的肿瘤抑制因子。然而，其在肾细胞癌中的功能尚未阐明。在本研究中，我们检测了肾细胞癌中miR-203a的表达，并评估了其与临床特征的关联。在肾细胞癌组织和肾细胞癌细胞中发现了miR-203a的过表达。高miR-203a表达与肿瘤分期和较短的总生存时间相关。生物信息学和荧光素酶测定证实糖原合酶激酶-3β是miR-203a的靶基因。沉默miR-203a可抑制细胞增殖和迁移，使它们停滞在G1期，并在体外促进细胞凋亡。miR-203a促进肾细胞癌的进展并预示预后不良。
