Department of Cardiology, Shengjing Hospital of China Medical University, Shenyang, China.
Department of Biotherapy, Cancer Research Institute, the First Affiliated Hospital, China Medical University, Shenyang, China.
Cell Cycle. 2022 Jul;21(14):1502-1511. doi: 10.1080/15384101.2022.2054250. Epub 2022 Apr 21.
Renal cell carcinoma (RCC) is one of the most frequent human tumors and has brought great threats to the health of the people around the globe. It was reported that linc01133, a long non-coding RNA (lncRNA), was involved in the pathogenesis and development of several human cancer. But the biological role of linc01133 in RCC is still not understood. The present study aimed to investigate the biological functions of linc01133 in RCC. We did some biological experiments in this study, including quantitative real-time polymerase chain reaction (qRT-PCR), western blotting, MTT assay, wound healing assay, Transwell invasion assay and xenograft tumor assay. In this study, we found the expression levels of linc01133 markedly increased in the RCC tissues compared with the normal tissues. And we found that the over-expressing of linc01133 promoted cell proliferation, migration and invasion, the interfering of linc01133 inhibited cell proliferation, migration and invasion. Furthermore, we found that the interfering of linc01133 inhibited tumor growth in murine xenograft models. Additionally, we found that linc01133 promotes RCC cell proliferation, migration and invasion through sponging miR-760. Collectively, our work preliminarily illuminated the tumor-promoting role of linc01133 in RCC and the potential molecular mechanism. Thus, our study may provide some evidence for the treatment of RCC.
肾细胞癌（RCC）是最常见的人类肿瘤之一，给全球人民的健康带来了巨大威胁。有报道称，长链非编码 RNA（lncRNA）linc01133 参与了几种人类癌症的发病机制和发展。但 linc01133 在 RCC 中的生物学作用尚不清楚。本研究旨在探讨 linc01133 在 RCC 中的生物学功能。我们在这项研究中进行了一些生物学实验，包括定量实时聚合酶链反应（qRT-PCR）、western blot、MTT 测定、划痕愈合测定、Transwell 侵袭测定和异种移植肿瘤测定。在这项研究中，我们发现 linc01133 的表达水平在 RCC 组织中明显高于正常组织。我们发现 linc01133 的过表达促进了细胞增殖、迁移和侵袭，而 linc01133 的干扰抑制了细胞增殖、迁移和侵袭。此外，我们发现 linc01133 的干扰抑制了小鼠异种移植模型中的肿瘤生长。此外，我们发现 linc01133 通过海绵吸附 miR-760 促进 RCC 细胞增殖、迁移和侵袭。总之，我们的工作初步阐明了 linc01133 在 RCC 中的促肿瘤作用及其潜在的分子机制。因此，我们的研究可能为 RCC 的治疗提供一些依据。
