Talebi Ali Reza, Mangoli Esmat, Nahangi Hossein, Anvari Morteza, Pourentezari Majid, Halvaei Iman
Research & Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Research & Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Eur J Obstet Gynecol Reprod Biol. 2014 Oct;181:32-6. doi: 10.1016/j.ejogrb.2014.07.007. Epub 2014 Jul 30.
The main goal was to examine the protective effects of vitamin C on sperm parameters, sperm chromatin condensation and apoptosis in experimentally-induced diabetic mice.
28 adult Syrian mice were divided into 4 groups. In Group1, the mice were diabetic that received a single dose of Streptozocin (STZ) (200mg/kg) intra-peritoneally (ip). Group 2 was included diabetic mice that received vitamin C (10mg/kg/daily, ip). Mice in group 3 were received vitamin C and group 4 was considered as control. After 35 days, sperm analysis was done accordingly. To asses sperm chromatin and DNA quality, we used aniline blue (AB), toluidine blue (TB), chromomycin A3 (CMA3), acridine orange (AO) and terminal transferase mediated deoxyuridine triphosphate biotin end labeling (TUNEL) tests.
All of the sperm parameters (count, motility, morphology and viability) had significant reduction in diabetic mice but, the data showed a significant increase in all of the sperm parameters in diabetic+vitamin C when compared with diabetic and control animals (P<0.05). There were significant differences (P<0.001) between groups regarding to TB staining (48.8±5.92 vs 34.3±4.13), AO test (35.9±6.11 vs 20.8±2.89) and TUNEL test (39.42±7.18 vs 22.00±3.65) in diabetic and diabetic+vitamin C groups, respectively. Nevertheless, in CMA3 and AB staining assays, there were not any significant differences between different groups.
Vitamin C, as a potent antioxidant, can attenuate detrimental effects of diabetes mellitus on the sperm parameters, chromatin quality and apoptosis in an experimental model.
主要目标是研究维生素C对实验性糖尿病小鼠精子参数、精子染色质凝聚及细胞凋亡的保护作用。
28只成年叙利亚小鼠被分为4组。第1组为糖尿病小鼠，腹腔注射一次链脲佐菌素（STZ）（200mg/kg）。第2组为接受维生素C（10mg/kg/每日，腹腔注射）的糖尿病小鼠。第3组小鼠接受维生素C，第4组作为对照。35天后，相应地进行精子分析。为评估精子染色质和DNA质量，我们使用了苯胺蓝（AB）、甲苯胺蓝（TB）、嗜铬霉素A3（CMA3）、吖啶橙（AO）及末端脱氧核苷酸转移酶介导的dUTP生物素末端标记（TUNEL）试验。
糖尿病小鼠的所有精子参数（数量、活力、形态及存活率）均显著降低，但数据显示，与糖尿病组和对照组动物相比，糖尿病+维生素C组的所有精子参数均显著增加（P<0.05）。糖尿病组和糖尿病+维生素C组在TB染色（48.8±5.92对34.3±4.13）、AO试验（35.9±6.11对20.8±2.89）及TUNEL试验（39.42±7.18对22.00±3.65）方面存在显著差异（P<0.001）。然而，在CMA3和AB染色试验中，不同组之间没有任何显著差异。
作为一种有效的抗氧化剂，维生素C可减轻糖尿病对实验模型中精子参数、染色质质量及细胞凋亡的有害影响。
