Sampannang Apichakan, Arun Supatcharee, Burawat Jaturon, Sukhorum Wannisa, Iamsaard Sitthichai
Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Department of Anatomy, School of Medicine, Mae Fah Luang University, Chiang Rai, Thailand.
Clin Exp Reprod Med. 2020 Mar;47(1):20-33. doi: 10.5653/cerm.2020.00388. Epub 2020 Feb 10.
The differences between type 1 and type 2 diabetes mellitus (T1DM and T2DM) in terms of their adverse effects on male reproductive parameters have never been elucidated. This study aimed to distinguish between the effects of the DM types in mice treated with multiple low doses of streptozotocin (STZ) to mimic human T1DM and coadministered a high-fat diet (HFD) to mimic human T2DM.
The T1DM mice were intraperitoneally injected with STZ (40 mg/kg body weight) for 5 days. The T2DM mice received an HFD for 14 days prior to STZ injection (85 mg/kg body weight), followed by continuous feeding of an HFD. Male reproductive parameters were evaluated.
The reproductive organs of the DM mice weighed significantly less than those of controls, and the seminal vesicles plus prostates of the T1DM mice weighed less than those of the T2DM mice. Increased sperm abnormalities and incomplete DNA packaging were observed in the DM groups. Sperm concentration and the proportion of normal sperm were significantly lower in the T1DM group. The seminiferous histopathology of DM mice was classified into seven types. The penises of the DM mice were smaller than those of the controls; however, tunica albuginea thickness and the amount of penile collagen fibers were increased in these mice. Round germ cells were abundant in the epididymal lumens of the mice with DM.
T1DM adversely affected reproductive parameters to a greater extent than T2DM.
1型和2型糖尿病（T1DM和T2DM）对男性生殖参数的不良影响差异尚未阐明。本研究旨在区分多次低剂量链脲佐菌素（STZ）处理以模拟人类T1DM并联合高脂饮食（HFD）以模拟人类T2DM的小鼠中糖尿病类型的影响。
T1DM小鼠腹腔注射STZ（40mg/kg体重），连续5天。T2DM小鼠在注射STZ（85mg/kg体重）前14天接受高脂饮食，随后持续给予高脂饮食。评估雄性生殖参数。
糖尿病小鼠的生殖器官重量明显低于对照组，T1DM小鼠的精囊加前列腺重量低于T2DM小鼠。糖尿病组观察到精子异常增加和DNA包装不完整。T1DM组的精子浓度和正常精子比例显著降低。糖尿病小鼠的生精组织病理学分为七种类型。糖尿病小鼠的阴茎比对照组小；然而，这些小鼠的白膜厚度和阴茎胶原纤维数量增加。糖尿病小鼠附睾管腔内圆形生殖细胞丰富。
T1DM对生殖参数的不良影响比T2DM更大。
