Chen Zhilei, Wang Zhenjun, Chen Jianjun, Li Zhulin
Clin Lab. 2018 Jan 1;64(1):25-31. doi: 10.7754/Clin.Lab.2017.170519.
Fibulin-5 has recently been considered as a potential tumor suppressor in human cancers. Several studies have shown that it is down-regulated in a variety of tumor types and inhibits tumor growth and metastasis. In this study, the expression of fibulin-5 in colorectal cancer (CRC) and its clinical significance were assessed.
Fibulin-5 expression was detected in 31 samples of surgically resected CRC and paired noncancerous tissues using western blot, qRT-PCR, and immunoblotting.
In this study, the expression levels of fibulin-5 protein and mRNA were down-regulated in CRC tissues as compared with those in paired noncancerous tissues. Low expression of fibulin-5 was significantly correlated with poor prognostic features including lymph node metastasis and advanced tumor-node-metastasis (TNM) tumor stage.
Fibulin-5 is down-regulated in CRC and the reduced expression of fibulin-5 was correlated with malignant clinicopathological characteristics.
最近，纤连蛋白-5被认为是人类癌症中一种潜在的肿瘤抑制因子。多项研究表明，它在多种肿瘤类型中表达下调，并能抑制肿瘤生长和转移。在本研究中，评估了纤连蛋白-5在结直肠癌（CRC）中的表达及其临床意义。
使用蛋白质免疫印迹法、定量逆转录聚合酶链反应（qRT-PCR）和免疫印迹法检测了31例手术切除的CRC样本及其配对的癌旁组织中纤连蛋白-5的表达。
在本研究中，与配对的癌旁组织相比，CRC组织中纤连蛋白-5蛋白和mRNA的表达水平下调。纤连蛋白-5低表达与包括淋巴结转移和晚期肿瘤-淋巴结-转移（TNM）肿瘤分期在内的不良预后特征显著相关。
纤连蛋白-5在CRC中表达下调，其表达降低与恶性临床病理特征相关。
