Stowe D J, Atkinson T, Mann N H
Department of Biological Sciences, University of Warwick, Coventry, UK.
Biochimie. 1989 Sep-Oct;71(9-10):1101-5. doi: 10.1016/0300-9084(89)90117-x.
Protein kinase activities were detected in cell-free extracts of the B385 derivative of Streptomyces coelicolor A3(2); at least 12 polypeptides, ranging in Mr from 6,000 to 98,000, were detectably phosphorylated, probably as O-monoesters, after incubation with gamma [32P]ATP. The culture stage of the mycelia used for production of the cell-free extracts determined the profile of phosphorylated polypeptides. Phosphoenol pyruvate acted as a potent modulator of the apparent degree of protein kinase activity. In addition Ca2+ ions, verapamil, chlorpromazine and anti-calmodulin antiserum had specific effects on the profile of phosphopolypeptides observed.
在天蓝色链霉菌A3(2)的B385衍生物的无细胞提取物中检测到蛋白激酶活性；与γ[32P]ATP孵育后，至少有12种分子量在6000至98000之间的多肽被检测到发生了磷酸化，可能是作为O-单酯。用于制备无细胞提取物的菌丝体的培养阶段决定了磷酸化多肽的图谱。磷酸烯醇丙酮酸作为蛋白激酶活性表观程度的有效调节剂。此外，Ca2+离子、维拉帕米、氯丙嗪和抗钙调蛋白抗血清对观察到的磷酸化多肽图谱有特定影响。
