Clough Bret H, McCarley Matthew R, Krause Ulf, Zeitouni Suzanne, Froese Jeremiah J, McNeill Eoin P, Chaput Christopher D, Sampson H Wayne, Gregory Carl A
Institute for Regenerative Medicine at Scott and White Hospital, Texas A&M Health Science Center, Temple, TX, USA.
J Bone Miner Res. 2015 Jan;30(1):83-94. doi: 10.1002/jbmr.2320.
Although bone has remarkable regenerative capacity, about 10% of long bone fractures and 25% to 40% of vertebral fusion procedures fail to heal. In such instances, a scaffold is employed to bridge the lesion and accommodate osteoprogenitors. Although synthetic bone scaffolds mimic some of the characteristics of bone matrix, their effectiveness can vary because of biological incompatibility. Herein, we demonstrate that a composite prepared with osteogenically enhanced mesenchymal stem cells (OEhMSCs) and their extracellular matrix (ECM) has an unprecedented capacity for the repair of critical-sized defects of murine femora. Furthermore, OEhMSCs do not cause lymphocyte activation, and ECM/OEhMSC composites retain their in vivo efficacy after cryopreservation. Finally, we show that attachment to the ECM by OEhMSCs stimulates the production of osteogenic and angiogenic factors. These data demonstrate that composites of OEhMSCs and their ECM could be utilized in the place of autologous bone graft for complex orthopedic reconstructions.
尽管骨骼具有显著的再生能力，但约10%的长骨骨折以及25%至40%的椎体融合手术无法愈合。在这种情况下，会使用支架来桥接损伤部位并容纳骨祖细胞。虽然合成骨支架模仿了骨基质的一些特性，但由于生物不相容性，它们的有效性可能会有所不同。在此，我们证明由成骨增强的间充质干细胞（OEhMSCs）及其细胞外基质（ECM）制备的复合材料具有前所未有的修复小鼠股骨临界尺寸缺损的能力。此外，OEhMSCs不会引起淋巴细胞激活，并且ECM/OEhMSC复合材料在冷冻保存后仍保留其体内疗效。最后，我们表明OEhMSCs对ECM的附着刺激了成骨和血管生成因子的产生。这些数据表明，OEhMSCs及其ECM的复合材料可用于替代自体骨移植进行复杂的骨科重建。
