Department of Biological Sciences and Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117543, Singapore.
Department of Biological Sciences and Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117543, Singapore.
Dev Cell. 2014 Aug 25;30(4):437-48. doi: 10.1016/j.devcel.2014.07.004. Epub 2014 Aug 14.
Plants adjust the timing of the transition to flowering to ensure their reproductive success in changing environments. Temperature and light are major environmental signals that affect flowering time through converging on the transcriptional regulation of FLOWERING LOCUS T (FT) encoding the florigen in Arabidopsis. Here, we show that a MYB transcription factor EARLY FLOWERING MYB PROTEIN (EFM) plays an important role in directly repressing FT expression in the leaf vasculature. EFM mediates the effect of ambient temperature on flowering and is directly promoted by another major FT repressor, SHORT VEGETATIVE PHASE. EFM interacts with an H3K36me2 demethylase JMJ30, which forms a negative feedback regulatory loop with the light-responsive circadian clock, to specifically demethylate an active mark H3K36me2 at FT. Our results suggest that EFM is an important convergence point that mediates plant responses to temperature and light to determine the timing of reproduction.
植物会调整向开花期过渡的时间，以确保其在不断变化的环境中繁殖成功。温度和光照是主要的环境信号，它们通过汇聚到调控开花时间的拟南芥 FLOWERING LOCUS T（FT）的转录水平上来影响开花时间。在这里，我们发现一个 MYB 转录因子 EARLY FLOWERING MYB PROTEIN（EFM）在直接抑制叶片维管束中 FT 表达方面发挥着重要作用。EFM 介导了环境温度对开花的影响，并直接受另一个主要的 FT 抑制因子 SHORT VEGETATIVE PHASE 的促进。EFM 与 H3K36me2 去甲基酶 JMJ30 相互作用，与光响应的生物钟形成负反馈调节环，特异性地在 FT 上去除活跃的 H3K36me2 标记。我们的结果表明，EFM 是一个重要的汇聚点，它介导了植物对温度和光照的反应，以确定繁殖的时间。
