Nenseth Hatice Z, Dezitter Xavier, Tesikova Martina, Mueller Florian, Klokk Tove I, Hager Gordon L, Saatcioglu Fahri
Department of Biosciences, University of Oslo, Oslo, Norway.
Computational Imaging and Modeling Unit, Institut Pasteur, Paris, France.
PLoS One. 2014 Aug 18;9(8):e105204. doi: 10.1371/journal.pone.0105204. eCollection 2014.
Closely related transcription factors (TFs) can bind to the same response elements (REs) with similar affinities and activate transcription. However, it is unknown whether transcription is similarly orchestrated by different TFs bound at the same RE. Here we have compared the recovery half time (t1/2), binding site occupancy and the resulting temporal changes in transcription upon binding of two closely related steroid receptors, the androgen and glucocorticoid receptors (AR and GR), to their common hormone REs (HREs). We show that there are significant differences at all of these levels between AR and GR at the MMTV HRE when activated by their ligands. These data show that two TFs bound at the same RE can have significantly different modes of action that can affect their responses to environmental cues.
密切相关的转录因子（TFs）能够以相似的亲和力结合相同的反应元件（REs）并激活转录。然而，尚不清楚由结合在同一RE上的不同TFs进行的转录调控是否类似。在此，我们比较了两种密切相关的类固醇受体，即雄激素受体和糖皮质激素受体（AR和GR），在结合其共同的激素反应元件（HREs）时的恢复半衰期（t1/2）、结合位点占有率以及转录过程中随之发生的时间变化。我们发现，当被其配体激活时，在MMTV HRE处，AR和GR在所有这些水平上均存在显著差异。这些数据表明，结合在同一RE上的两种TFs可以具有显著不同的作用模式，这可能会影响它们对环境信号的反应。
