含5-（羟甲基）尿嘧啶的DNA对原核和真核DNA及RNA聚合酶的模板引物活性。

Template-primer activity of 5-(hydroxymethyl)uracil-containing DNA for prokaryotic and eukaryotic DNA and RNA polymerases.

作者信息

Herrala A M, Vilpo J A

机构信息

Laboratory of Molecular Hematology, University of Oulu, Finland.

出版信息

Biochemistry. 1989 Oct 17;28(21):8274-7. doi: 10.1021/bi00447a003.

DOI:10.1021/bi00447a003

PMID:2513877

Abstract

We have utilized Bacillus subtilis phage SPO-1 DNA as a model of irradiated DNA. In this phage, all thymine (Thy) residues are replaced by 5-(hydroxymethyl)uracil (5HmUra), which is a known irradiation-induced derivative of DNA Thy. SPO-1 phage is naturally devoid of other such irradiation-induced DNA lesions. DNase I activated SPO-1 phage DNA served as well as, or even better than, the control DNAs (Bacillus subtilis DNA and calf thymus DNA) as a template-primer for Escherichia coli, Micrococcus luteus, and human HL-60 cell DNA polymerases. Furthermore, the template activity of SPO-1 phage DNA was also superior when transcription with E. coli RNA polymerase was investigated. The results reported here indicated that the replacement of Thy by 5HmUra is not deleterious to template and primer functions during DNA or RNA synthesis.

摘要

我们利用枯草芽孢杆菌噬菌体SPO-1 DNA作为辐照DNA的模型。在这种噬菌体中，所有胸腺嘧啶（Thy）残基都被5-（羟甲基）尿嘧啶（5HmUra）取代，5HmUra是一种已知的DNA胸腺嘧啶的辐照诱导衍生物。SPO-1噬菌体天然不存在其他此类辐照诱导的DNA损伤。经DNase I激活的SPO-1噬菌体DNA作为大肠杆菌、藤黄微球菌和人HL-60细胞DNA聚合酶的模板引物，其效果与对照DNA（枯草芽孢杆菌DNA和小牛胸腺DNA）相当，甚至更好。此外，在研究用大肠杆菌RNA聚合酶进行转录时，SPO-1噬菌体DNA的模板活性也更优。此处报道的结果表明，用5HmUra取代胸腺嘧啶在DNA或RNA合成过程中对模板和引物功能无害。